Complete Nucleotide Sequences of the Domestic Cat (Felis catus) Mitochondrial Genome and a Transposed mtDNA Tandem Repeat (Numt) in the Nuclear Genome

Authors

• Jose V. Lopez, National Cancer Institute - FCRDC; George Mason UniversityFollow
• Stanley Cevario, National Cancer Institute-FCRDC
• Stephen J. O'Brien, National Cancer Institute - FCRDC

ORCID

0000-0002-1637-4125; 0000-0001-7353-8301

ResearcherID

F-8809-2011; N-1726-2015

Document Type

Article

Publication Title

Genomics

ISSN

0888-7543

Publication Date

4-1996

Keywords

Amino acid sequence, Animals, Base sequence, Cats/genetics, Cell nucleus, DNA transposable elements, Mitochondrial DNA, Genome, Humans, Mitochondria, Molecular sequence data, Nucleic acid conformation, Phylogeny, Repetitive sequences, Nucleic acid

Abstract

The complete 17,009-bp mitochondrial genome of the domestic cat, Felis catus, has been sequenced and conforms largely to the typical organization of previously characterized mammalian mtDNAs. Codon usage and base composition also followed canonical vertebrate patterns, except for an unusual ATC (non-AUG) codon initiating the NADH dehydrogenase subunit 2 (ND2) gene. Two distinct repetitive motifs at opposite ends of the control region contribute to the relatively large size (1559 bp) of this carnivore mtDNA. Alignment of the feline mtDNA genome to a homologous 7946-bp nuclear mtDNA tandem repeat DNA sequence in the cat, Numt, indicates simple repeat motifs associated with insertion/deletion mutations. Overall DNA sequence divergence between Numt and cytoplasmic mtDNA sequence was only 5.1%. Substitutions predominate at the third codon position of homologous feline protein genes. Phylogenetic analysis of mitochondrial gene sequences confirms the recent transfer of the cytoplasmic mtDNA sequences to the domestic cat nucleus and recapitulates evolutionary relationships between mammal species.

DOI

10.1006/geno.1996.0188

Volume

33

Issue

2

First Page

229

Last Page

246

Comments

©1996 Academic Press. All rights reserved.

NSUWorks Citation

Lopez, Jose V.; Stanley Cevario; and Stephen J. O'Brien. 1996. "Complete Nucleotide Sequences of the Domestic Cat (Felis catus) Mitochondrial Genome and a Transposed mtDNA Tandem Repeat (Numt) in the Nuclear Genome." Genomics 33, (2): 229-246. doi:10.1006/geno.1996.0188.