Title

Vibrio caribbeanicus sp. nov., Isolated from the Marine Sponge Scleritoderma cyanea

Authors

Maria Hoffman, United States Food and Drug Administration
Steven R. Monday, United States Food and Drug Administration
Marc W. Allard, United States Food and Drug Administration
Errol A. Strain, United States Food and Drug Administration
Paul Whittaker, United States Food and Drug Administration
Marianna Naum, United States Food and Drug Administration
Peter J. McCarthy, United States Food and Drug Administration
Jose V. Lopez, Nova Southeastern UniversityFollow
Markus Fischer, University of Hamburg - Germany
Eric W. Brown, United States Food and Drug Administration

Document Type

Article

Publication Date

8-2012

Publication Title

International Journal of Systematic and Evolutionary Microbiology

Keywords

Bacterial typing techniques, Base composition, Bacterial DNA, Fatty acids analysis, Bacterial genes, Likelihood functions, Molecular sequence data, Porifera, RNA, 16S genetics, DNA sequence analysis, Vibrio classification, Vibrio isolation and purification

ISSN

1466-5026

Volume

62

Issue/No.

8

First Page

1736

Last Page

1743

Abstract

A Gram-negative, oxidase-positive, catalase-negative, facultatively anaerobic, motile, curved rod-shaped bacterium, strain N384^T, was isolated from a marine sponge (Scleritoderma cyanea; phylum Porifera) collected from a depth of 795 feet (242 m) off the west coast of Curaçao. On the basis of 16S rRNA gene sequencing, strain N384^Twas shown to belong to the genus Vibrio, most closely related to Vibrio brasiliensis LMG 20546^T (98.8 % similarity), Vibrio nigripulchritudo ATCC 27043^T (98.5 %), Vibrio tubiashii ATCC 19109^T (98.6 %) and V. sinaloensis DSM 21326^T (98.2 %). The DNA G+C content of strain N384^Twas 41.6 mol%. An analysis of concatenated sequences of five genes (gyrB,rpoA, pyrH, mreB and ftsZ; 4068 bp) demonstrated a clear separation between strain N384^T and its closest neighbours and clustered strain N384^T into the ‘Orientalis’ clade of vibrios. Phenotypically, the novel species belonged to the arginine dihydrolase-positive, lysine decarboxylase- and ornithine decarboxylase-negative (A+/L−/O−) cluster. The novel species was also differentiated on the basis of fatty acid composition, specifically that the proportions of iso-C_13 : 0, iso-C_15 : 0, C_15 : 0, iso-C_16 : 0, C_16 : 0, iso-C_17 : 0, C_17 : 1ω8c and C_17 : 0 were significantly different from those found in V. brasiliensisand V. sinaloensis. The results of DNA–DNA hybridization, average nucleotide identity and physiological and biochemical tests further allowed differentiation of this strain from other described species of the genus Vibrio. Collectively, these findings confirm that strain N384^T represents a novel Vibrio species, for which the name Vibrio caribbeanicus sp. nov. is proposed, with the type strain N384^T ( = ATCC BAA-2122^T = DSM 23640^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene, topA,ftsZ, gapA, gyrB, mreB, pyrH, recA and rpoA gene sequences derived in this study are HM771333–HM771388 and HQ890462–HQ890466. The accession numbers for the sequences of strain N384^T are GU223601 (16S rRNA), HM771337 (topA), HM771358 (ftsZ), HM771363 (gapA), HM771368 (gyrB), HM771373 (mreB), HM771378 (pyrH), HM771383 (recA), HM771388 (rpoA) and AEIU00000000 (whole-genome shotgun sequence). The accession numbers for the whole-genome shotgun sequences of V. brasiliensis LMG 20546^T, V. sinaloensis DSM 21326^T, V. splendidus ATCC 33789, V. ichthyoenteri ATCC 700023^T, V. scophthalmi LMG 19158^T, V. orientalis ATCC 33934^T, V. tubiashii ATCC 19109^T and V. nigripulchritudo ATCC 27043^T are AEVS00000000, AEVT00000000, AFWG00000000, AFWF00000000, AFWE00000000, AFWH00000000, AFWI00000000 and AFWJ00000000.

Comments

©2012 IUMS

NSUWorks Citation

Hoffman, Maria; Steven R. Monday; Marc W. Allard; Errol A. Strain; Paul Whittaker; Marianna Naum; Peter J. McCarthy; Jose V. Lopez; Markus Fischer; and Eric W. Brown. 2012. "Vibrio caribbeanicus sp. nov., Isolated from the Marine Sponge Scleritoderma cyanea." International Journal of Systematic and Evolutionary Microbiology 62, (8): 1736-1743. doi:10.1099/ijs.0.032375-0.

ORCID ID

0000-0002-1637-4125

ResearcherID

F-8809-2011

DOI

10.1099/ijs.0.032375-0