Title

De Novo Sequencing, Assembly and Analysis of Eight Different Transcriptomes from the Malayan Pangolin

Authors

Aini Mohamed Yusoff, University of Malaya - Kuala Lumpur, Malaysia
Tze King Tan, University of Malaya - Kuala Lumpur, Malaysia
Ranjeev Hari, University of Malaya - Kuala Lumpur, Malaysia
Klaus-Peter Koepfli, Smithsonian Conservation Biology Institute
Wei Yee Wee, University of Malaya - Kuala Lumpur, Malaysia
Agostinho Antunes, Universidade do Porto - Portugal
Frankie Thomas Sitam, Department of Wildlife and National Parks - Malaysia
Jeffrine Japning Rovie-Ryan, Department of Wildlife and National Parks - Malaysia
Kayal Vizi Karuppannan, Department of Wildlife and National Parks - Malaysia
Guat Jah Wong, University of Malaya - Kuala Lumpur, Malaysia
Leonard Lipovich, Wayne State University
Wesley C. Warren, Washington University in St. Louis
Stephen J. O'Brien, St. Petersburg State University - Russia; Nova Southeastern UniversityFollow
Siew Woh Choo, University of Malaya - Kuala Lumpur, Malaysia

Document Type

Article

Publication Date

9-13-2016

Publication Title

Scientific Reports

ISSN

2045-2323

Volume

6

Issue/No.

28199

First Page

1

Last Page

11

Abstract

Pangolins are scale-covered mammals, containing eight endangered species. Maintaining pangolins in captivity is a significant challenge, in part because little is known about their genetics. Here we provide the first large-scale sequencing of the critically endangered Manis javanica transcriptomes from eight different organs using Illumina HiSeq technology, yielding ~75 Giga bases and 89,754 unigenes. We found some unigenes involved in the insect hormone biosynthesis pathway and also 747 lipids metabolism-related unigenes that may be insightful to understand the lipid metabolism system in pangolins. Comparative analysis between M. javanica and other mammals revealed many pangolin-specific genes significantly over-represented in stress-related processes, cell proliferation and external stimulus, probably reflecting the traits and adaptations of the analyzed pregnant female M. javanica. Our study provides an invaluable resource for future functional works that may be highly relevant for the conservation of pangolins.

Comments

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Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

NSUWorks Citation

Mohamed Yusoff, Aini; Tze King Tan; Ranjeev Hari; Klaus-Peter Koepfli; Wei Yee Wee; Agostinho Antunes; Frankie Thomas Sitam; Jeffrine Japning Rovie-Ryan; Kayal Vizi Karuppannan; Guat Jah Wong; Leonard Lipovich; Wesley C. Warren; Stephen J. O'Brien; and Siew Woh Choo. 2016. "De Novo Sequencing, Assembly and Analysis of Eight Different Transcriptomes from the Malayan Pangolin." Scientific Reports 6, (28199): 1-11. doi:10.1038/srep28199.

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

DOI

10.1038/srep28199