Title

Conservation Genetics of the Koala (Phascolarctos cinereus) : Low Mitochondrial DNA Variation Amongst Southern Australian Populations

Authors

Andrea C. Taylor, National Cancer Institute at Frederick
Jenny Marshall Graves, National Cancer Institute at Frederick
Neil D. Murray, National Cancer Institute at Frederick
Stephen J. O'Brien, National Cancer Institute at FrederickFollow
Naoya Yuhki, National Cancer Institute at Frederick
Bill Sherwin, National Cancer Institute at Frederick

Document Type

Article

Publication Date

2-1997

Publication Title

Genetics Research

ISSN

0016-6723

Volume

69

Issue/No.

1

First Page

25

Last Page

33

Abstract

Koala (Phascolarctos cinereus) populations in southern Australia have a history of bottlenecks – earlier this century the species became extinct in South Australia, and almost so in Victoria. Subsequently large numbers of animals from island populations (founded from very few animals) have been translocated back to mainland sites and to other islands in the region. As part of a larger study of the genetic structure of koala populations in southern Australia, we have undertaken a survey of mitochondrial DNA restriction fragment length polymorphism (mtDNA-RFLP) variability. Genomic DNA from 91 koalas from five populations was examined using 23 restriction enzymes, and mtDNA fragments were detected using a domestic cat full-length mtDNA clone. Only one of the enzymes, TaqI, revealed polymorphism – a relatively low amount of variation compared with other mammals, although low mtDNA-RFLP variation has also been reported in Queensland koalas. French Island and populations established predominantly from French Island immigrant koalas, either directly or via other island populations, were indistinguishable by haplotype frequencies. The mtDNA data are thus consistent with the interpretation that the koala translocation programme has homogenized gene frequencies amongst those populations involved. South Gippsland is not recorded as having received translocated koalas directly, and has significantly different mtDNA-RFLP haplotype frequencies from all other populations examined. The fact that this distinction was not previously observed in nuclear gene frequencies may reflect predominantly male-mediated dispersal in koalas.

Comments

©1997 Cambridge University Press

NSUWorks Citation

Taylor, Andrea C.; Jenny Marshall Graves; Neil D. Murray; Stephen J. O'Brien; Naoya Yuhki; and Bill Sherwin. 1997. "Conservation Genetics of the Koala (Phascolarctos cinereus) : Low Mitochondrial DNA Variation Amongst Southern Australian Populations." Genetics Research 69, (1): 25-33. https://nsuworks.nova.edu/cnso_bio_facarticles/706

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015