Title

Quantitative Polymerase Chain Reaction-based Assay for Estimating DNA Yield Extracted from Domestic Cat Specimens

Authors

Marilyn Menotti-Raymond, National Cancer Institute at Frederick
Victor David, National Cancer Institute at Frederick
Leslie Wachter, National Cancer Institute at Frederick
Naoya Yuhki, National Cancer Institute at Frederick
Stephen J. O'Brien, National Cancer Institute at FrederickFollow

Document Type

Article

Publication Date

6-2003

Publication Title

Croatian Medical Journal

Keywords

Cats, Forensic medicine, Polymerase chain reaction, Short interspersed nucleotide elements, Tandem repeat sequences

ISSN

0353-9504

Volume

44

Issue/No.

3

First Page

327

Last Page

331

Abstract

A quantitative polymerase chain reaction (PCR) assay has been developed for the quantification of genomic DNA extracted from domestic cat samples. The assay, which targets highly repetitive genomic short interspersed nuclear elements (SINE), can be performed rapidly and is highly sensitive, detecting as little as 10 fg of feline genomic DNA. The assaywas linear over a 10⁶ dilution range.Wehave recently developed a short tandem repeat (STR) multiplex panel for forensic analysis of feline specimens. The SINE assay is an integral part of the forensic typing system. The sensitivity of the assaywill enable forensic examiners to determine the likelihood of success of genotyping sample extractswith the STR panel without sacrificing valuable DNA necessary to perform genotyping of samples.

NSUWorks Citation

Menotti-Raymond, Marilyn; Victor David; Leslie Wachter; Naoya Yuhki; and Stephen J. O'Brien. 2003. "Quantitative Polymerase Chain Reaction-based Assay for Estimating DNA Yield Extracted from Domestic Cat Specimens." Croatian Medical Journal 44, (3): 327-331. https://nsuworks.nova.edu/cnso_bio_facarticles/630

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015