Comparative Feline Genomics: A BAC/PAC Contig Map of the Major Histocompatibility Complex Class II Region

Authors

• Thomas W. Beck, National Cancer Institute at Frederick
• Joan C. Menninger, National Cancer Institute at Frederick
• Ginny Voigt, National Cancer Institute at Frederick
• Kimberly Newmann, National Cancer Institute at Frederick
• Yoko Nishigaki, National Cancer Institute at Frederick
• William G. Nash, H&W Cytogenetic Services, Inc.
• Robert M. Stephens, National Cancer Institute at Frederick
• Yu Wang, Children's Hospital Oakland Research Institute
• Pieter J. de Jong, Children's Hospital Oakland Research Institute
• Stephen J. O'Brien, National Cancer Institute at FrederickFollow
• Naoya Yuhki, National Cancer Institute at Frederick

ORCID

0000-0001-7353-8301

ResearcherID

N-1726-2015

Document Type

Article

Publication Title

Genomics

ISSN

0888-7543

Publication Date

2-1-2001

Abstract

The genome organization of the human major histocompatibility complex (MHC) will be best understood in a comparative evolutionary context. We describe here the construction of a physical map for the feline MHC. A large-insert domestic cat genomic DNA library was developed using a P1 artificial chromosome (PAC) with a genomic representation of 2.53 and an average insert size of 80 kb. A sequence-ready 660-kb bacterial artificial chromosome/PAC contig map of the domestic cat MHC class II region was constructed with a gene order similar to, but distinct from, that of human and mice: DPB/DPA, Ring3, DMB, TAP1, DOB, DRB2, DRA3, DRB1, DRA2, and DRA1. Fluorescence in situ hybridization analyses of selected class II PAC clones confirmed that the class II region lies in the pericentromeric region of cat chromosome B2. However, apparently unlike the human and mouse MHCs, the domestic cat DRA and DRB genes have undergone multiple duplications and the DQ region has been deleted.

Volume

72

Issue

3

First Page

282

Last Page

295

Comments

©2001 Academic Press

NSUWorks Citation

Beck, Thomas W.; Joan C. Menninger; Ginny Voigt; Kimberly Newmann; Yoko Nishigaki; William G. Nash; Robert M. Stephens; Yu Wang; Pieter J. de Jong; Stephen J. O'Brien; and Naoya Yuhki. 2001. "Comparative Feline Genomics: A BAC/PAC Contig Map of the Major Histocompatibility Complex Class II Region." Genomics 72, (3): 282-295. https://nsuworks.nova.edu/cnso_bio_facarticles/620