Biology Faculty Articles
Title
Comparative Feline Genomics: A BAC/PAC Contig Map of the Major Histocompatibility Complex Class II Region
Document Type
Article
Publication Date
2-1-2001
Publication Title
Genomics
ISSN
0888-7543
Volume
72
Issue/No.
3
First Page
282
Last Page
295
Abstract
The genome organization of the human major histocompatibility complex (MHC) will be best understood in a comparative evolutionary context. We describe here the construction of a physical map for the feline MHC. A large-insert domestic cat genomic DNA library was developed using a P1 artificial chromosome (PAC) with a genomic representation of 2.53 and an average insert size of 80 kb. A sequence-ready 660-kb bacterial artificial chromosome/PAC contig map of the domestic cat MHC class II region was constructed with a gene order similar to, but distinct from, that of human and mice: DPB/DPA, Ring3, DMB, TAP1, DOB, DRB2, DRA3, DRB1, DRA2, and DRA1. Fluorescence in situ hybridization analyses of selected class II PAC clones confirmed that the class II region lies in the pericentromeric region of cat chromosome B2. However, apparently unlike the human and mouse MHCs, the domestic cat DRA and DRB genes have undergone multiple duplications and the DQ region has been deleted.
NSUWorks Citation
Beck, Thomas W.; Joan C. Menninger; Ginny Voigt; Kimberly Newmann; Yoko Nishigaki; William G. Nash; Robert M. Stephens; Yu Wang; Pieter J. de Jong; Stephen J. O'Brien; and Naoya Yuhki. 2001. "Comparative Feline Genomics: A BAC/PAC Contig Map of the Major Histocompatibility Complex Class II Region." Genomics 72, (3): 282-295. https://nsuworks.nova.edu/cnso_bio_facarticles/620
ORCID ID
0000-0001-7353-8301
ResearcherID
N-1726-2015
Comments
©2001 Academic Press