Biology Faculty Articles

Title

Development and Characterization of Microsatellite Loci in the Endangered Oyster Mussel Epioblasma capsaeformis (Bivalvia: Unionidae)

Document Type

Article

Publication Date

12-2004

Publication Title

Molecular Ecology Notes

Keywords

DNA, Epioblasma, Freshwater mussel, Microsatellites

ISSN

1471-8278

Volume

4

Issue/No.

4

First Page

649

Last Page

652

Abstract

Primers for 10 polymorphic microsatellite loci were developed and characterized for the endangered oyster mussel Epioblasma capsaeformis from the Clinch River, Tennessee. Microsatellite loci also were tested in four other populations or species. Amplification was successful for most loci in these closely related endangered species or populations; therefore, a high level of flanking sequence similarity was inferred for this group of species and populations. Allelic diversity ranged from nine to 20 alleles/locus, and averaged 13.6/locus. This study demonstrated the feasibility of using polymerase chain reaction (PCR) primers to amplify microsatellite loci across freshwater mussel species to conduct population genetics studies.

Comments

©2004 Blackwell Publishing Ltd

Additional Comments

GenBank accession #s: AY650389-AY650398

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

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