Biology Faculty Articles
Title
Comparative Genomic Structure of Human, Dog, and Cat MHC: HLA, DLA, and FLA
Document Type
Article
Publication Date
7-2007
Publication Title
Journal of Heredity
ISSN
0022-1503
Volume
98
Issue/No.
5
First Page
390
Last Page
399
Abstract
Comparisons of the genomic structure of 3 mammalian major histocompatibility complexes (MHCs), human HLA, canine DLA, and feline FLA revealed remarkable structural differences between HLA and the other 2 MHCs. The 4.6-Mb HLA sequence was compared with the 3.9-Mb DLA sequence from 2 supercontigs generated by 7x whole-genome shotgun assembly and 3.3-Mb FLA draft sequence. For FLA, we confirm that 1) feline FLA was split into 2 pieces within the TRIM (member of the tripartite motif) gene family found in human HLA, 2) class II, III, and I regions were placed in the pericentromeric region of the long arm of chromosome B2, and 3) the remaining FLA was located in subtelomeric region of the short arm of chromosome B2. The exact same chromosome break was found in canine DLA structure, where class II, III, and I regions were placed in a pericentromeric region of chromosome 12 whereas the remaining region was located in a subtelomeric region of chromosome 35, suggesting that this chromosome break occurred once before the split of felid and canid more than 55 million years ago. However, significant differences were found in the content of genes in both pericentromeric and subtelomeric regions in DLA and FLA, the gene number, and amplicon structure of class I genes plus 2 other class I genes found on 2 additional chromosomes; canine chromosomes 7 and 18 suggest the dynamic nature in the evolution of MHC class I genes.
Additional Comments
National Cancer Institute contract #: N01-CO-12400
NSUWorks Citation
Yuhki, Naoya; Thomas W. Beck; R. Stephens; Beena A. Neelam; and Stephen J. O'Brien. 2007. "Comparative Genomic Structure of Human, Dog, and Cat MHC: HLA, DLA, and FLA." Journal of Heredity 98, (5): 390-399. https://nsuworks.nova.edu/cnso_bio_facarticles/502
ORCID ID
0000-0001-7353-8301
ResearcherID
N-1726-2015
Comments
©2007 Oxford University Press