Title

Organization and Chromosomal Specificity of Autosomal Homologs of Human Y Chromosome Repeated DNA

Authors

Robert D. Burk, Johns Hopkins University
Paul Szabo, Sloan Kettering Institute
Stephen J. O'Brien, National Cancer Institute at FrederickFollow
William G. Nash, National Cancer Institute at Frederick
Lohchung Yu, Lawrence Livermore Laboratory
Kirby D. Smith, Johns Hopkins University

Document Type

Article

Publication Date

6-1985

Publication Title

Chromosoma

ISSN

0009-5915

Volume

92

Issue/No.

3

First Page

225

Last Page

233

Abstract

The human Y chromosome contains a group of repeated DNA elements, identified as 3.4-kilobase pair (kb) fragments in Hae III digests of male genomic DNA, which contain both Y-specific and non-Y-specific sequences. We have used these 3.4-kb Hae III Y fragments to explore the organizational properties and chromosomal distribution of the autosomal homologs of the non-Y-specific (NYS) 3.4-kb Hae III Y elements. Three distinct organizations, termed domains, have been identified and shown to have major concentrations on separate chromosomes. We have established that domain K is located on chromosome 15 and domain D on chromosome 16 and suggested that domain R is on chromosome 1. Our findings suggest that each domain is composed of a tandemly arrayed cluster of a regularly repeating unit containing two sets of repeated sequences: one that is homologous to the NYS 3.4-kb Hae III Y sequences and one that does not cross-react with the 3.4-kb Hae III Y repeats. Thus, these autosomal repeated DNA domains, like their Y chromosome counterparts, consist of a complex mixture of repeated DNA elements interspersed among each other in ways that lead to defined periodicities. Although each of the three identified autosomal domains cross-reacts with 3.4-kb Hae III Y fragments purified from genomic DNA, the length periodicities and sequence content of the autosomal domains are chromosome specific. The organizational properties and chromosomal distribution of these NYS 3.4-kb Hae III homologs seem inconsistent with stochastic mechanisms of sequence diffusion between chromosomes.

Comments

©1985 Springer-Verlag

NSUWorks Citation

Burk, Robert D.; Paul Szabo; Stephen J. O'Brien; William G. Nash; Lohchung Yu; and Kirby D. Smith. 1985. "Organization and Chromosomal Specificity of Autosomal Homologs of Human Y Chromosome Repeated DNA." Chromosoma 92, (3): 225-233. https://nsuworks.nova.edu/cnso_bio_facarticles/391

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015