Cytogenetic Methodologies for Gene Mapping and Comparative Analyses in Mammalian Cell Culture Systems

Authors

• William Modi, National Cancer Institute at Frederick
• William Nash, National Cancer Institute at Frederick
• Anna C. Ferrari, National Cancer Institute at Frederick; Union Memorial Hospital
• Stephen J. O'Brien, National Cancer Institute at FrederickFollow

ORCID

0000-0001-7353-8301

ResearcherID

N-1726-2015

Document Type

Article

Publication Title

Gene Analysis Techniques

ISSN

0735-0651

Publication Date

1987

Abstract

Presented here are the detailed methods employed in our laboratory for gene mapping and cytogenetic analyses in human beings, in the domestic cat, and in other mammalian species. Included in the procedures are: 1) establishment of primary fibroblast and lymphoid cell cultures; 2) heterologous cell fusion for production of rapidly proliferating cell hybrids; 3) cellular transformation of primary fibroblasts using an oncogenic retrovirus; 4) cell synchronization for high-resolution banding of prometaphase chromosomes; 5) chromosome-banding procedures, including G-banding, alkaline G-11, and Q-banding; and 6) in situ hybridization of radiolabeled molecular clones to metaphase chromosomes for regional gene localization.

Volume

4

Issue

4

First Page

75

Last Page

85

Comments

© 1987 Published by Elsevier B.V.

NSUWorks Citation

Modi, William; William Nash; Anna C. Ferrari; and Stephen J. O'Brien. 1987. "Cytogenetic Methodologies for Gene Mapping and Comparative Analyses in Mammalian Cell Culture Systems." Gene Analysis Techniques 4, (4): 75-85. https://nsuworks.nova.edu/cnso_bio_facarticles/369