Cloning and Comparative Mapping of a Human Class III (X) Alcohol Dehydrogenase cDNA

Authors

• P. Rathna Giri, National Institute on Alcohol Abuse and Alcoholism
• Jane F. Krug, National Institute on Alcohol Abuse and Alcoholism
• Christine Kozak, National Institute of Allergy and Infectious Diseases
• Tamyra Moretti, National Institute on Alcohol Abuse and Alcoholism
• Stephen J. O'Brien, National Cancer Institute at FrederickFollow
• Hector Seuanez, National Cancer Institute at Frederick
• David Goldman, National Institute on Alcohol Abuse and Alcoholism

ORCID

0000-0001-7353-8301

ResearcherID

N-1726-2015

Document Type

Article

Publication Title

Biochemical and Biophysical Research Communications

ISSN

0006-291X

Publication Date

10-16-1989

Abstract

A cDNA encoding human class III (χ, ADH5) alcohol dehydrogenase was isolated, sequenced and used to comparatively map this unusual ADH. In their coding sequences, the three major ADH classes were approximately equisimilar, class II and III ADHs sharing the highest sequence identity (67%). A class III-like ADH was mapped to mouse chromosome 3, site of the ADH gene complex, and synteny of ADH5 with four other ADH loci on human chromosome 4 was confirmed. The nearly full-length 1613 nucleotide cDNA contained 433 nucleotides of 3′ nontranslated sequence and two possible initiation sites for translation. A protein of 374 amino acid residues could be synthesized using the potential initiation codon at nucleotide 59. However, use of the likely initiation codon at nucleotide 5 would produce a protein of 392 residues with 19 additional N-terminal residues as compared to the known protein sequence. The derived protein sequence also differs at residue 166, where Tyr is found. This difference, due to a single base substitution, could result from cloning artifact, polymorphism, or two expressed class III ADH genes.

Volume

164

Issue

1

First Page

453

Last Page

460

Comments

© 1989 Published by Elsevier Inc.

NSUWorks Citation

Giri, P. Rathna; Jane F. Krug; Christine Kozak; Tamyra Moretti; Stephen J. O'Brien; Hector Seuanez; and David Goldman. 1989. "Cloning and Comparative Mapping of a Human Class III (X) Alcohol Dehydrogenase cDNA." Biochemical and Biophysical Research Communications 164, (1): 453-460. https://nsuworks.nova.edu/cnso_bio_facarticles/347