Biology Faculty Articles
Title
An Oncogenic Chromosome 8-9 Gene Fusion Isolated Following Transfection of Human Ovarian Carcinoma Cell Line DNA
Document Type
Article
Publication Date
7-1990
Publication Title
Oncogene
ISSN
0950-9232
Volume
5
Issue/No.
7
First Page
1085
Last Page
1089
Abstract
Transfection of NIH3T3 cells with genomic DNA from the human ovarian adenocarcinoma tumor cell line OVCAR-3 identified ovc, a rearranged human DNA sequence which was generated during transfection and which induced both morphological transformation and tumorigenesis. A human alu repeat positive 10.5 kb EcoRI fragment present in all transformants was cloned, and two alu-free fragments of 1.8 kb and 2.2 kb were subcloned. The cloned 10.5 kb fragment is not biologically active in DNA transfection assays. Probe from the 2.2 kb fragment hybridizes to poly A + RNA from the transformants and several human tumor cell lines, including OVCAR-3. The 2.2 kb fragment maps to a site on human chromosome 9 (9p24) not known to contain oncogenic sequences, and identifies a two allele polymorphic restriction site. The 1.8 kb fragment maps to human chromosome 8. The ovc transforming sequences fail to hybridize to probes to any of 14 known oncogenes, indicating that they may represent a previously unknown human transforming gene.
Additional Comments
National Cancer Institute contract #s: N01-CO-74101, N01-CO-74102
NSUWorks Citation
Halverson, D.; W. S. Modi; M. Dean; E. P. Gelmann; K. J. Dunn; D. Clanton; M. Oskarsson; Stephen J. O'Brien; and D. G. Blair. 1990. "An Oncogenic Chromosome 8-9 Gene Fusion Isolated Following Transfection of Human Ovarian Carcinoma Cell Line DNA." Oncogene 5, (7): 1085-1089. https://nsuworks.nova.edu/cnso_bio_facarticles/338
ORCID ID
0000-0001-7353-8301
ResearcherID
N-1726-2015
Comments
©1990 Nature Publishing Group