Influence of Temperature and Gas Atmosphere on In-Vitro Fertilization and Embryo Development in Domestic Cats

Authors

• L. A. Johnston, National Zoological Park - Washington, D.C.; National Cancer Institute at Frederick
• A. M. Donoghue, National Zoological Park - Washington, D.C.
• Stephen J. O'Brien, National Cancer Institute at FrederickFollow
• David E. Wildt, National Zoological Park - Washington, D.C.

ORCID

0000-0001-7353-8301

ResearcherID

N-1726-2015

Document Type

Article

Publication Title

Journal of Reproduction and Fertility

ISSN

0022-4251

Publication Date

7-1-1991

Keywords

Cat, Fertilization, Embryo, In vitro culture, Incubation, Temperature, Gas atmosphere

Abstract

The influence of culture temperature and gas atmosphere on in-vitro fertilization and embryo development was examined in the domestic cat. In Exp. 1, eggs were fertilized and cultured in 5% CO₂ in air at 37, 38 or 39°C. Experiment 2 evaluated the effects of 5% CO₂ in air; 5% CO₂, 5% O₂ and 90% N₂; and 10% CO₂ in air. Fertilization (cleavage) and development to the morula/blastocyst stage were not influenced (P > 0·05) by variations in temperature and gas composition. Despite changing these culture conditions, egg cleavage averaged ∼75% and >80% of the 2-cell embryos proceeded to morulae in vitro. However, the partial in-vitro morula-to-blastocyst developmental block normally observed in this species was not removed.

Volume

92

Issue

2

First Page

377

Last Page

382

Comments

©1991 Journals of Reproduction and Fertility Ltd

Additional Comments

Nationa Institutes of Health grant #: NIH HD 23583

NSUWorks Citation

Johnston, L. A.; A. M. Donoghue; Stephen J. O'Brien; and David E. Wildt. 1991. "Influence of Temperature and Gas Atmosphere on In-Vitro Fertilization and Embryo Development in Domestic Cats." Journal of Reproduction and Fertility 92, (2): 377-382. https://nsuworks.nova.edu/cnso_bio_facarticles/330