Title

Chromosomal Localization of Nucleic Acid-Binding Proteins by Affinity Mapping: Assignment of the IRE-Binding Protein Gene to Human Chromosome 9

Authors

Matthias W. Hentze, National Institute of Child Health and Human Development
Hector Seuanez, National Cancer Institute at Frederick
Stephen J. O'Brien, National Cancer Institute at FrederickFollow
Joe B. Harford, National Institute of Child Health and Human Development
Richard D. Klausner, National Institute of Child Health and Human Development

Document Type

Article

Publication Date

8-11-1989

Publication Title

Nucleic Acids Research

ISSN

0305-1048

Volume

17

Issue/No.

15

First Page

6103

Last Page

6108

Abstract

Three human mRNAs are regulated post-transcriptionally by iron via iron-responsive elements (IREs) contained in each mRNA. A cytoplasmic protein (IRE-BP) binds to these cis-acting elements and mediates the translational regulation of ferritin H- and L-chain mRNA and the iron-dependent stability of transferrin receptor (TfR) mRNA. We have taken advantage of the different mobilities of the human and rodent IRE/IRE-BP complexes on non-denaturing polyacrylamide gels to determine the chromosomal localization of the gene encoding the IRE-BP. Utilizing a panel of 34 different human/rodent hybrid cell lines we have assigned the IRE-BP gene to human chromosome 9. This new technique based on nucleic acid/protein interaction may allow determination of the chromosomal localization of other RNA- or DNA-binding proteins.

Comments

©1989 IRL Press

NSUWorks Citation

Hentze, Matthias W.; Hector Seuanez; Stephen J. O'Brien; Joe B. Harford; and Richard D. Klausner. 1989. "Chromosomal Localization of Nucleic Acid-Binding Proteins by Affinity Mapping: Assignment of the IRE-Binding Protein Gene to Human Chromosome 9." Nucleic Acids Research 17, (15): 6103-6108. https://nsuworks.nova.edu/cnso_bio_facarticles/300

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015