Title

Influence of Culture Medium and Protein Supplementation on In Vitro Oocyte Maturation and Fertilization in the Domestic Cat

Authors

L. A. Johnston, Smithsonian Institution; Henry Doorly Zoo; National Cancer Institute at Frederick
A. M. Donoghue, Smithsonian Institution
Stephen J. O'Brien, National Cancer Institute at FrederickFollow
David E. Wildt, Smithsonian Institution

Document Type

Article

Publication Date

10-1993

Publication Title

Theriogenology

Keywords

Cat, Oocyte maturation, Fertilization, Embryo

ISSN

0093-691X

Volume

40

Issue/No.

4

First Page

829

Last Page

839

Abstract

Domestic cat oocytes were cultured either in Waymouth MB 753/1 Medium (WAY) or in Eagle's Minimum Essential Medium (MEM) containing FSH, LH and estradiol-17β and supplemented with one of the following: 5% fetal calf serum (FCS); 4 mg/ml bovine serum albumin (BSA); or 3 mg/ml polyvinylalcohol (PVA, a non-protein control). The oocytes were evaluated for: nuclear maturation after 48 hours of culture (in vitro maturation, IVM); fertilization and cleavage 24 to 30 hours postinsemination (in vitro fertilization, IVF); and early embryo development 48 hours postinsemination. Maturation rates were similar (P>0.05) for WAY + BSA (29.4%), MEM + BSA (46.7%) and MEM + PVA (43.3%), but were different (P0.05) for WAY + BSA (51.4%, 30.5%), MEM + BSA (45.8%, 40.1%) and MEM + PVA (56.1%, 37.4%) and were greater (Pin vitro can proceed in this species in the absence of supplementary protein.

Comments

©1993 Butterworth-Heinemann

NSUWorks Citation

Johnston, L. A.; A. M. Donoghue; Stephen J. O'Brien; and David E. Wildt. 1993. "Influence of Culture Medium and Protein Supplementation on In Vitro Oocyte Maturation and Fertilization in the Domestic Cat." Theriogenology 40, (4): 829-839. https://nsuworks.nova.edu/cnso_bio_facarticles/276

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015