Biology Faculty Articles

Title

Genetic Characterization of Parental Cell Lines and Biochemical Analysis of Somatic Cell Hybrids Between Mouse (RAG) Cells and Fibroblasts of Ateles paniscus chamek (Primates, Platyrrhini)

Document Type

Article

Publication Date

1993

Publication Title

American Journal of Primatology

Keywords

Ateles, Hybrid cell lines, Chromosomes, Biochemical markers

ISSN

0275-2565

Volume

30

Issue/No.

3

First Page

181

Last Page

194

Abstract

Mouse (RAG) cells, (deficient in hypoxanthine-phosphoribosyl-transferase), and Ateles paniscus chamek primary fibroblasts were used in fusion experiments to generate somatic cell hybrids. Both parental cell lines were genetically characterized by karyological and biochemical analyses with 27 isozyme systems. These procedures were useful for monitoring primate chromosome segregation in somatic cell hybrids, for detecting chromosome rearrangements of primate chromosomes, and for identifying individual primate chromosomes.

These characterizations are necessary to distinguish between different hybrid cell lines and to generate a panel for gene mapping studies. This is achieved by selecting cell lines that segregate different sets of relatively few primate isozymes and chromosomes. Conversely, we eliminated hybrid cell lines either showing: (1) rearrangements between primate and mouse chromosomes, (2) extensive rearrangements of primate chromosomes, or (3) a large number of primate biochemical markers.

Comments

© 1993 Wiley-Liss, Inc.

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

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Peer Reviewed

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