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Biology Faculty Articles
Title
Assignment of Islet Amyloid Polypeptide (IAPP) Gene to Feline Chromosome B4 Using the Polymerase Chain Reaction Technique on Feline-Rodent Hybrid Cell Lines
Document Type
Article
Publication Date
3-1995
Publication Title
Veterinary Pathology
Keywords
Chromosome, Diabetes mellitus, Islet amyloid polypeptide, Polymerase chain reaction
ISSN
0300-9858
Volume
32
Issue/No.
2
First Page
195
Last Page
197
Abstract
The most characteristic morphologic features of the pancreatic islets of human non-insulin-dependent diabetes mellitus (NIDDM, or type 2 diabetes mellitus) and of similar forms of diabetes in cats and macaques are the deposition of amyloid (islet amyloid) and the loss of beta cells. Islet amyloid is derived from islet amyloid polypeptide (IAPP), which is a normal secretory product of the beta cells. Therefore, knowledge of the IAPP gene is of potential importance in defining the pathogenesis of NIDDM. To identify the feline chromosome(s) on which the IAPP gene is located, we screened genomic DNA obtained from 38 feline-rodent hybrid cell lines that have a known feline chromosome content. Feline IAPP DNA was amplified and detected using the polymerase chain reaction technique. Discordancy analysis for each feline chromosome showed that chromosome B4 had the lowest discordancy (P < 0.0001). Feline chromosome B4 shows an extensive conserved syntenic relationship with human chromosome 12, on which the human IAPP gene is located. This study therefore extends and confirms the homology between human chromosome 12 and feline chromosome B4 and provides an additional genetic marker for feline chromosome B4.
Additional Comments
National Institute of Diabetes and Digestive and Kidney Disease grant #:R01 DK36734
NSUWorks Citation
Jordan, K.; Stephen J. O'Brien; K. H. Johnson; and T. D. O'Brien. 1995. "Assignment of Islet Amyloid Polypeptide (IAPP) Gene to Feline Chromosome B4 Using the Polymerase Chain Reaction Technique on Feline-Rodent Hybrid Cell Lines." Veterinary Pathology 32, (2): 195-197. https://nsuworks.nova.edu/cnso_bio_facarticles/259
ORCID ID
0000-0001-7353-8301
ResearcherID
N-1726-2015
Comments
©1995 by American College of Veterinary Pathologists