Title

The ets Sequence from the Transforming Gene of Avian Erythroblastosis Virus, E26, has Unique Domains on Human Chromosomes 11 and 21: Both Loci are Transcriptionally Active

Authors

Dennis K. Watson, National Cancer Institute at Frederick
Mary J. McWilliams-Smith, National Cancer Institute at Frederick
M. F. Nunn, Salk Institute
Peter Duesberg, University of California at Berkeley
Stephen J. O'Brien, National Cancer Institute at FrederickFollow
Takis S. Papas, National Cancer Institute at Frederick

Document Type

Article

Publication Date

11-1-1985

Publication Title

Proceedings of the National Academy of Sciences of the United States of America

Keywords

Acute leukemia, Oncogene expression

ISSN

1091-6490

Volume

82

Issue/No.

21

First Page

7294

Last Page

7298

Abstract

Human DNA segments homologous to the ets region from the transforming gene of avian erythroblastosis virus, E26, were molecularly cloned and shown to be closely related to the viral equivalent by hybridization and partial sequence analysis. The transforming gene of E26 has a tripartite origin with the structure ∆gag [1.2 kilobases (kb) from the viral gag gene]-myb(0.9 kb from the chicken myb gene)-ets (1.6 kb from the chicken ets gene). Human ets DNA is located on two distinct human chromosomes. The human ets-1 locus on chromosome 11 encodes a single mRNA of 6.8 kb; the second locus, ets-2 on chromosome 21, encodes three mRNAs of 4.7, 3.2, and 2.7 kb. The ets-related sequences of human DNA on chromosomes 11 and 21 are discontiguous, except for a small overlap region encoding 14 amino acids, where 12 are conserved between these two loci. By contrast, the chicken homolog has contiguous ets-1 and ets-2 sequences and is primarily expressed in normal chicken cells as a single 7.5-kb mRNA. We conclude that the ets sequence shared by the virus, the chicken, and humans is likely to contain at least two dissociable functional domains, ets-1 and ets-2. Thus, the tripartite transforming gene of E26 includes four distinct domains that may be functionally relevant for the transforming function of the virus (∆gag, myb, ets-1, and ets-2).

NSUWorks Citation

Watson, Dennis K.; Mary J. McWilliams-Smith; M. F. Nunn; Peter Duesberg; Stephen J. O'Brien; and Takis S. Papas. 1985. "The ets Sequence from the Transforming Gene of Avian Erythroblastosis Virus, E26, has Unique Domains on Human Chromosomes 11 and 21: Both Loci are Transcriptionally Active." Proceedings of the National Academy of Sciences of the United States of America 82, (21): 7294-7298. https://nsuworks.nova.edu/cnso_bio_facarticles/243

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015