Title

Bvr-1, a Restriction Locus of a Type C RNA Virus in the Feline Cellular Genome: Pleiotropic Restriction of Endogenous BALB Virus in Cat x Mouse Somatic Cell Hybrids

Authors

Stephen J. O'Brien, National Cancer Institute, BethesdaFollow
Janice M. Simonson, National Cancer Institute at Bethesda

Document Type

Article

Publication Date

1-1-1978

Publication Title

Journal of Experimental Medicine

ISSN

0022-1007

Volume

147

Issue/No.

1

First Page

219

Last Page

232

Abstract

Bvr-1 is a dominant X-linked feline gene which restricts the replication of B-tropic murineleukemia virus (B-MuLV) in somatic cell hybrids between murine BALB/c-RAG cells and FL-74 feline cells. Since the hybrids were originally derived by the hypoxanthine aminopterin thymidine selection scheme, counter selection experiments on 6-thioguanine result in preferential survival of hybrid cells which have spontaneously lost the feline X-chromosome on which is located the structural gene for hypoxanthine guanine phosphoribosyl transferase (IMP: pyrophosphate phosphoribosyl transferase, E.C. 2.4.2.8) and Bvr-1. Back selected Bvr-1^- cells express high parental levels of B-MuLV. Bvr-1^- effectively restricts the IdU-mediated induction of the endogenous xenotropic BALB virus (BALB: virus 2) but not the endogenous N-tropic virus (BALB: virus 1). Pleiotropic restriction of B-MuLV and X-MuLV, but not N-MuLV suggests that the viral targets of Bvr-1 (either viral components or functions in viral assembly) of the B-tropic and X-tropic endogenous BALB viruses are similar to each other but distinct from the target in the N-tropic virus. Very low levels of B-MuLV are detected in restricted cells, but this residual virus is not infectious in either NIH-3T3 or BALB-3T3 mouse cells which are genotypically Fv-1^N/Fv-1^N and Fv-1^B/Fv-1^B, respectively. Passage of residual virus through host cells without Fv-1 related restriction (SC-1) results in production of infectious B-MuLV indistinguishable from that produced by RAG parent cells.

Comments

© 1978 Rockefeller University Press

NSUWorks Citation

O'Brien, Stephen J. and Janice M. Simonson. 1978. "Bvr-1, a Restriction Locus of a Type C RNA Virus in the Feline Cellular Genome: Pleiotropic Restriction of Endogenous BALB Virus in Cat x Mouse Somatic Cell Hybrids." Journal of Experimental Medicine 147, (1): 219-232. doi:10.1084/jem.147.1.219.

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

DOI

10.1084/jem.147.1.219