Enzyme Polymorphisms as Genetic Signatures in Human Cell Cultures

Authors

• Stephen J. O'Brien, National Cancer Institute at BethesdaFollow
• Gail Kleiner, National Cancer Institute at Bethesda
• Russell Olson, Flow Laboratories
• John E. Shannon, American Type Culture Collection

ORCID

0000-0001-7353-8301

ResearcherID

N-1726-2015

Document Type

Article

Publication Title

Science

ISSN

0036-8075

Publication Date

3-25-1977

Abstract

The electrophoretic resolution of seven relatively polymorphic human gene-enzyme systems expressed in tissue culture cells can be used as a sensitive genetic monitor for intraspecific cell contamination. An identical genotype at each of the same allozyme loci provides a 95 percent (or greater) confidence estimate of the identity of two cultured lines, on the basis of the allelic frequencies of the seven enzyme loci in natural populations and in populations of independently derived cultured cells. Of 27 commonly used human cell lines examined, only one of 351 pairwise comparisons proved genetically indistinguishable.

DOI

10.1126/science.841332

Volume

195

Issue

4284

First Page

1345

Last Page

1348

Comments

©1977 American Association for the Advancement of Science

NSUWorks Citation

O'Brien, Stephen J.; Gail Kleiner; Russell Olson; and John E. Shannon. 1977. "Enzyme Polymorphisms as Genetic Signatures in Human Cell Cultures." Science 195, (4284): 1345-1348. doi:10.1126/science.841332.