Biology Faculty Articles
Title
BAC-end Sequence Analysis and a Draft Physical Map of the Common Bean (Phaseolus vulgaris L.) Genome
Document Type
Article
Publication Date
1-15-2008
Publication Title
Tropical Plant Biology
Keywords
BAC-end sequence, Phaseolus vulgaris, Physical map
ISSN
19359764
Volume
1
First Page
40
Last Page
48
Abstract
Common bean (Phaseolus vulgaris L.) is a legume that is an important source of dietary protein in developing countries throughout the world. Utilizing the G19833 BAC library for P. vulgaris from Clemson University, 89,017 BAC-end sequences were generated giving 62,588,675 base pairs of genomic sequence covering approximately 9.54% of the genome. Analysis of these sequences in combination with 1,404 shotgun sequences from the cultivar Bat7 revealed that approximately 49.2% of the genome contains repetitive sequence and 29.3% is genic. Compared to other legume BAC-end sequencing projects, it appears that P. vulgaris has higher predicted levels of repetitive sequence, but this may be due to a more intense identification strategy combining both similarity-based matches as well as de novo identification of repeats. In addition, fingerprints for 41,717 BACs were obtained and assembled into a draft physical map consisting of 1,183 clone contigs and 6,385 singletons with ~9x coverage of the genome.
NSUWorks Citation
Schlueter, Jessica A.; Jose Luis Goicoechea; Kristi Collura; Navdeep Gill; Jer-Young Lin; Yeisoo Yu; Dave Kudrna; Andrea Zuccolo; C. Eduardo Vallejos; Monica Muñoz-Torres; Matthew W. Blair; Joe Tohme; Jeff Tomkins; Phillip McClean; Rod A. Wing; and Scott A. Jackson. 2008. "BAC-end Sequence Analysis and a Draft Physical Map of the Common Bean (Phaseolus vulgaris L.) Genome." Tropical Plant Biology 1, (): 40-48. doi:10.1007/s12042-007-9003-9.
ORCID ID
http://orcid.org/0000-0003-3746-1866
DOI
10.1007/s12042-007-9003-9
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