Biology Faculty Articles
Document Type
Article
Publication Date
9-28-2021
Publication Title
mSystems
Keywords
Pseudomonas aeruginosa, pyoverdine, biofilms, disturbance, mathematical modeling, virulence factors
ISSN
2379-5077
Volume
6
Issue/No.
5
First Page
e00961-21
Abstract
Understanding the environmental factors that affect the production of virulence factors has major implications in evolution and medicine. While spatial structure is important in virulence factor production, observations of this relationship have occurred in undisturbed or continuously disturbed environments. However, natural environments are subject to periodic fluctuations, including changes in physical forces, which could alter the spatial structure of bacterial populations and impact virulence factor production. Using Pseudomonas aeruginosa PA14, we periodically applied a physical force to biofilms and examined production of pyoverdine. Intermediate frequencies of disturbance reduced the amount of pyoverdine produced compared to undisturbed or frequently disturbed conditions. To explore the generality of this finding, we examined how an intermediate disturbance frequency affected pyoverdine production in 21 different strains of P. aeruginosa. Periodic disturbance increased, decreased, or did not change the amount of pyoverdine produced relative to undisturbed populations. Mathematical modeling predicts that interactions between pyoverdine synthesis rate and biofilm density determine the amount of pyoverdine synthesized. When the pyoverdine synthesis rates are high, depletion of the biofilm due to disturbance reduces the accumulation of pyoverdine. At intermediate synthesis rates, production of pyoverdine increases during disturbance as bacteria dispersed into the planktonic state enjoy increased growth and pyoverdine production rates. At low synthesis rates, disturbance does not alter the amount of pyoverdine produced since disturbance-driven access to nutrients does not augment pyoverdine synthesis. Our results suggest that environmental conditions shape robustness in the production of virulence factors and may lead to novel approaches to treat infections.
Additional Comments
© 2021 Quinn et al.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
NSUWorks Citation
Quinn, Rebecca J.; Ivana Barraza; Laura García-Diéguez; Camryn Pajon; Lauren E. Krausfeldt; Kerollos Ibrahim; Laura A. Enzinna; Morgan E. Thorn; Omar Tonsi Eldakar; Travis J. A. Craddock; and Robert P. Smith. 2021. "Periodically Disturbing the Spatial Structure of Biofilms Can Affect the Production of an Essential Virulence Factor in Pseudomonas aeruginosa." mSystems 6, (5): e00961-21. doi:10.1128/mSystems.00961-21.
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Supplemental Figure 7
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Supplementary Data
msystems.00961-21-st002.docx (14 kB)
Supplementary Data
msystems.00961-21-st003.docx (21 kB)
Supplementary Data
ORCID ID
0000-0001-7405-427X, 0000-0003-2744-7390, 0000-0002-4807-4979
DOI
10.1128/mSystems.00961-21
Comments
Research was sponsored by the Army Research Office and was accomplished under grant W911NF-18-1-0443. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of the Army Research Office or the U.S. Government. The U.S. Government is authorized to reproduce and distribute reprints for Government purposes notwithstanding any copyright notation here.