ddPCR Reveals SARS-CoV-2 Variants in Florida Wastewater

Authors

• Eben Gering, Nova Southeastern UniversityFollow
• Jacob Colbert, Nova Southeastern University
• Sarah Schmedes, Florida Department of Health
• George Duncan, Nova Southeastern UniversityFollow
• Jose V. Lopez, Nova Southeastern UniversityFollow
• Jessy Motes, Florida Department of Health
• James Weiss, Florida Department of Health
• Taj Azarian, Florida Department of Health
• Omer Tekin, Florida Department of Health
• Jason Blanton, Florida Department of Health

ORCID

0000-0002-4931-5316, 0000-0002-4931-5316, 0000-0002-1637-4125

ResearcherID

J-8619-2012, F-8809-2011

Document Type

Article

Publication Title

medRxiv

Publication Date

4-13-2021

Abstract

Wastewater was screened for the presence of functionally significant mutations in SARS-CoV-2 associated with emerging variants of concern (VOC) by ddPCR, and results accorded with sequencing of clinical samples from the same region. We propose that PCR-based screening of wastewater can provide a powerful tool for rapid and inexpensive screening of large population segments for VOC-associated mutations and can hone complementary sampling and sequencing of direct (human) test material to track emerging VOC.

DOI

10.1101/2021.04.08.21255119

First Page

21255119

Comments

Funding was provided via a CDC epidemiology and laboratory capacity grant (ELC).

Additional Comments

The copyright holder for this preprint is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.

NSUWorks Citation

Gering, Eben; Jacob Colbert; Sarah Schmedes; George Duncan; Jose V. Lopez; Jessy Motes; James Weiss; Taj Azarian; Omer Tekin; and Jason Blanton. 2021. "ddPCR Reveals SARS-CoV-2 Variants in Florida Wastewater." medRxiv , (): 21255119. doi:10.1101/2021.04.08.21255119.