Title

Microbial Community Shift Under Exposure of Dredged Sediments From a Eutrophic Bay

Authors

Juliana R. Nascimento, Universidade Federal Fluminense - Brazil
Cole Easson, Nova Southeastern University; Middle Tennessee State UniversityFollow
Diogo de A. Jurelevicius, Universidade Federal do Rio de Janeiro - Brazil
Jose V. Lopez, Nova Southeastern UniversityFollow
Edison D. Bidone, Universidade Federal Fluminense - Brazil
Elisamara Sabadini-Santos, Universidade Federal Fluminense - Brazil

Document Type

Article

Publication Date

7-23-2020

Publication Title

Environmental Monitoring and Assessment

Keywords

Guanabara Bay, Risk assessment, Quality ratio, Enzymes, Sequencing

ISSN

0167-6369

Volume

192

First Page

539

Abstract

Microbial communities occur in almost every habitat. To evaluate the homeostasis disruption of in situ microbiomes, dredged sediments from Guanabara Bay-Brazil (GB) were mixed with sediments from outside of the bay (D) in three different proportions (25%, 50%, and 75%) which we called GBD25, GBD50, and GBD75. Grain size, TOC, and metals—as indicators of complex contamination—dehydrogenase (DHA) and esterase enzymes (EST)—as indicators of microbial community availability—were determined. Microbial community composition was addressed by amplifying the 16S rRNA gene for DGGE analysis and sequencing using MiSeq platform (Illumina).We applied the quality ratio index (QR) to the GB, D, and every GBD mixture to integrate geochemical parameters with our microbiome data. QR indicated high environmental risk for GB and every GBD mixture, and low risk for D. The community shifted from aerobic to anaerobic profile, consistent with the characteristics of GB. Sample D was dominated by JTB255 marine benthic group, related to low impacted areas. Milano-WF1B-44 was the most representative of GB, often found in anaerobic and sulfur enriched environments. In GBD, the denitrifying sulfur-oxidizing bacteria, Sulfurovum, was the most representative, typically found in suboxic or anoxic niches. The canonical correspondence analysis was able to explain 60% of the community composition variation and exhibit the decrease of environmental quality as the contamination increases. Physiological and taxonomic shifts of the microbial assemblage in sediments were inferred by QR, which was suitable to determine sediment risk. The study produced sufficient information to improve the dredging plan and management.

Comments

©Springer Nature Switzerland AG 2020

Additional Comments

Programa de Doutorado Sanduíche no Exterior #: 88881.135581/2016-01; Conselho Nacional de Desenvolvimento Científico e Tecnológico grant #s: CNPq/Universal 449631/2014-1, CNPq/PDJ 155406/ 2018-3)

NSUWorks Citation

Nascimento, Juliana R.; Cole Easson; Diogo de A. Jurelevicius; Jose V. Lopez; Edison D. Bidone; and Elisamara Sabadini-Santos. 2020. "Microbial Community Shift Under Exposure of Dredged Sediments From a Eutrophic Bay." Environmental Monitoring and Assessment 192, (): 539. doi:10.1007/s10661-020-08507-8.

ORCID ID

0000-0002-1637-4125

ResearcherID

F-8809-2011

DOI

10.1007/s10661-020-08507-8