Biology Faculty Articles
Title
The Inoculum Effect and Band-Pass Bacterial Response to Periodic Antiobiotic Treatment
Document Type
Article
Publication Date
10-9-2012
Publication Title
Molecular Systems Biology
Keywords
Antibiotic, Bacteria, Bistability, Inoculum Effect
ISSN
1744-4292
Volume
8
Issue/No.
1
First Page
1
Last Page
11
Abstract
The inoculum effect (IE) refers to the decreasing efficacy of an antibiotic with increasing bacterial density. It represents a unique strategy of antibiotic tolerance and it can complicate design of effective antibiotic treatment of bacterial infections. To gain insight into this phenomenon, we have analyzed responses of a lab strain of Escherichia coli to antibiotics that target the ribosome. We show that the IE can be explained by bistable inhibition of bacterial growth. A critical requirement for this bistability is sufficiently fast degradation of ribosomes, which can result from antibiotic-induced heat-shock response. Furthermore, antibiotics that elicit the IE can lead to ‘band-pass’ response of bacterial growth to periodic antibiotic treatment: the treatment efficacy drastically diminishes at intermediate frequencies of treatment. Our proposed mechanism for the IE may be generally applicable to other bacterial species treated with antibiotics targeting the ribosomes.
Additional Comments
Highlighted in "Editor's Choice": Microbiology - Hit'Em Quick, Hit'Em Strong (2012) Science, 338, 6104_x000D_ _x000D_ Highlighted in Durham Herald Sun_x000D_ _x000D_ Highlighted in Sharkbytes and Duke University Biomedical Engineering Research Article
NSUWorks Citation
Tan, Cheemang; Robert P. Smith; Jaydeep K. Srimani; Katherine A. Riccione; Sameer Prasada; Meta Kuehn; and Lingchong You. 2012. "The Inoculum Effect and Band-Pass Bacterial Response to Periodic Antiobiotic Treatment." Molecular Systems Biology 8, (1): 1-11. https://nsuworks.nova.edu/cnso_bio_facarticles/6
Comments
Article #617