Title

The GLI-Kruppel Family of Human Genes

Authors

John M. Ruppert, Johns Hopkins University School of Medicine
Kenneth W. Kinzler, Johns Hopkins University School of Medicine
Albert J. Wong, Johns Hopkins University School of Medicine
Sandra H. Bigner, Duke University Medical Center
Fa-Ten Kao, University of Colorado - Denver
Martha L. Law, University of Colorado - Denver
Hector Seuanez, National Cancer Institute at Frederick
Stephen J. O'Brien, National Cancer Institute at FrederickFollow
Bert Vogelstein, Johns Hopkins University School of Medicine

Document Type

Article

Publication Date

8-1988

Publication Title

Molecular and Cellular Biology

ISSN

0270-7306

Volume

8

Issue/No.

8

First Page

3104

Last Page

3113

Abstract

Previous characterization of GLI, a gene found to be amplified and expressed in a subset of human brain tumors, revealed the presence of five tandem zinc fingers related to those of Krüppel (Kr), a Drosophila segmentation gene of the gap class. We have used the GLI cDNA as a molecular probe to isolate related sequences from the human genome. Partial characterization of six related loci, including sequence determination, expression studies, and chromosome localization, revealed that each locus could encode a separate finger protein. The predicted proteins all had similar H-C links, i.e., a conserved stretch of 9 amino acids connecting the C-terminal histidine of one finger to the N-terminal cysteine of the next. On the basis of amino acid sequence and intron-exon organization, the genes could be placed into one of two subgroups: the GLI subgroup (with the consensus finger amino acid sequence [Y/F]XCX₃GCX₃[F/Y]X₅LX₂HX_3-4H[T/S]GEKP) or the Kr subgroup (with the consensus finger amino acid sequence [Y/F]XCX₂CX₃FX₅LX₂HXRXHTGEKP). Unlike GLI or Kr, most of the newly isolated genes were expressed in many adult tissues. The predicted proteins probably control the expression of other genes and, by analogy with Kr and GLI, may be important in human development, tissue-specific differentiation, or neoplasia.

Comments

©1988, American Society for Microbiology

Additional Comments

National Cancer Institute grant #s: GM-07184, CA-09243; National Institutes of Health grant #s: CA-43722, NS-20023, GM-33093, HD-02080, HD-17449, CA-43460

NSUWorks Citation

Ruppert, John M.; Kenneth W. Kinzler; Albert J. Wong; Sandra H. Bigner; Fa-Ten Kao; Martha L. Law; Hector Seuanez; Stephen J. O'Brien; and Bert Vogelstein. 1988. "The GLI-Kruppel Family of Human Genes." Molecular and Cellular Biology 8, (8): 3104-3113. https://nsuworks.nova.edu/cnso_bio_facarticles/147

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015