Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures
Ultrastructural and Histological Analysis of Dark Spot Syndrome in Siderastrea siderea and Agaricia agaricites
11th International Coral Reef Symposium, Fort Lauderdale, FL, July 7-11, 2008
Dark Spot Syndrome, Siderastrea siderea, Agaricia agaricites, Ultrastructure, TEM
Dark Spot Syndrome (DSS) typically manifests in scleractinian corals as lesions of varying color, size, shape and location that can result in skeletal changes and tissue death. A causative agent for DSS has not yet been identified. The objective of this study was histological and ultrastructural comparison of the cellular and skeletal characteristics of DSS-affected and healthy Siderastrea siderea and Agaricia agaricites. The greater resolution possible with transmission electron microscopy (TEM) revealed microbial activity and tissue changes not resolvable utilizing histology. DSS-affected tissue had less integrity, with increasing cellular degradation and vacuolization. A high concentration of electron dense inclusions, which appear to be zymogen granules, was concentrated in the calicodermis and adjacent gastrodermal layer. Numerous endolithic fungal cells were observed directly adjacent to the calicodermis in DSS-affected S. siderea. Numerous unidentified endolithic cells were observed directly adjacent to the calicodermis in DSS-affected A. agaricites. These observations suggest that the coral may be using a digestive enzyme as a defensive mechanism against endolithic cellular invasion.
Renegar, Dorothy-Ellen A.; Blackwelder, Patricia; Miller, J. D.; Gochfeld, D. J.; and Moulding, Alison L., "Ultrastructural and Histological Analysis of Dark Spot Syndrome in Siderastrea siderea and Agaricia agaricites" (2008). Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures. 174.
Funding for this study was provided by Nova Southeastern University President’s Faculty Research & Development Grant #35369 to PLB, ALM, DAR and DJG, NOAA/National Institute for Undersea Science and Technology Grant #NA16RU1496 to DJG and NOAA/Ocean Exploration Grant #NA060AR4600184 To DJG.