Marine & Environmental Sciences Faculty Articles
Title
Passive Dosing Yields Dissolved Aqueous Exposures of Crude Oil Comparable to the CROSERF (Chemical Response to Oil Spill: Ecological Effects Research Forum) Water Accommodated Fraction Method
Document Type
Article
Publication Date
11-2018
Publication Title
Environmental Toxicology and Chemistry
Keywords
Water accommodated fraction, Passive dosing, Crude oil, PETROTOX
ISSN
0730-7268
Volume
37
Issue/No.
11
First Page
2810
Last Page
2819
Abstract
The Chemical Response to Oil Spill: Ecological Effects Research Forum's water accommodated fraction procedure was compared with 2 alternative techniques in which crude oil was passively dosed from silicone tubing or O‐rings. Fresh Macondo oil (MC252) was dosed at 30 mg/L using each approach to investigate oil dissolution kinetics, which was monitored by fluorometry as estimated oil equivalents (EOEs). Subsequent experiments with each dosing method were then conducted at multiple oil loadings. Following equilibration, test media were analytically characterized for polyaromatic hydrocarbons (PAHs) using gas chromatography (GC)–mass spectrometry and dissolved oil using biomimetic solid‐phase microextraction (SPME). The results showed that equilibrium was achieved within 72 h for all methods. Measured PAH concentrations were compared with oil solubility model predictions of dissolved exposures. The concentration and composition of measured and predicted dissolved PAHs varied with oil loading and were consistent between dosing methods. Two‐dimensional GC compositional data for this oil were then used to calculate dissolved toxic units for predicting MC252 oil acute toxicity across the expected range of species sensitivities. Predicted toxic units were nonlinear with loading and correlated to both EOE and biomimetic SPME. Passive dosing methods provide a practical strategy to deliver and maintain dissolved oil concentrations while avoiding the complicating role that droplets can introduce in exposure characterization and test interpretation.
NSUWorks Citation
Gopal Bera, Thomas Parkerton, Aaron Redman, Nicholas Turner, Dorothy-Ellen A. Renegar, Jose Sericano, and Anthony H. Knap. 2018. Passive Dosing Yields Dissolved Aqueous Exposures of Crude Oil Comparable to the CROSERF (Chemical Response to Oil Spill: Ecological Effects Research Forum) Water Accommodated Fraction Method .Environmental Toxicology and Chemistry , (11) : 2810 -2819. https://nsuworks.nova.edu/occ_facarticles/989.
ORCID ID
0000-0003-1330-1278
DOI
10.1002/etc.4263
Comments
©2018 SETAC