Marine & Environmental Sciences Faculty Articles

Title

Effect of Closed v. Intermittent‐Flow Respirometry on Hypoxia Tolerance in the Shiner Perch Cymatogaster aggregata

Document Type

Article

Publication Date

1-2016

Publication Title

Journal of Fish Biology

Keywords

Aquatic breathers, Critical oxygen saturation level, Hypoxic, Physiology, Respiration, Stress

ISSN

1095-8649

Volume

88

Issue/No.

1

First Page

252

Last Page

264

Abstract

This study compares the critical oxygen saturation (O2 crit) levels of the shiner perch Cymatogaster aggregata obtained using two different methods wherein hypoxia is induced either by the fish's respiration (closed respirometry) or by degassing oxygen with nitrogen (intermittent‐flow respirometry). Fish exhibited loss of equilibrium at a higher O2 saturation in the closed respirometry method when compared with the intermittent‐flow method. Utilization of closed respirometry yielded O2 crit measurements that were almost twice as high as those obtained with intermittent‐flow respirometry. The lower hypoxia tolerance in closed respirometry is consistent with additional stress, caused by a build‐up of ammonia and carbon dioxide and a faster rate in dissolved oxygen decline. The results indicate that these two methods of determining hypoxia tolerance in aquatic organisms are not comparable, and that much care should be given to method choice.

Comments

© 2016 The Fisheries Society of the British Isles

Additional Comments

NSF grant #: DGE-1144086

ORCID ID

0000-0001-8225-8344

DOI

10.1111/jfb.12837

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Peer Reviewed

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