Presentation Title

Development of an In Vitro Adhesion Test to Screen Mucoadhesive Polymeric Drug Carriers Using Texture Analyzer

Speaker Credentials

Ph.D. in Pharmacy

Speaker Credentials

PharmD

College

College of Pharmacy

Location

Nova Southeastern University, Davie, Florida, USA

Format

Poster

Start Date

21-2-2020 8:30 AM

End Date

21-2-2020 4:00 PM

Abstract

Objective:Developing an in vitro adhesion test to evaluate mucoadhesive oral films using texture analyzer. Background:Adhesive polymeric excipients are the integral part of many oral and topical dosage forms. These are used to increase retention of the drug at the site of application or in the gastrointestinal tract enhancing drug absorption and half-life. Ex vivo tests on animal tissues are commonly used to characterize mucoadhesion, however it is hard and costly. To differentiate adhesive properties of such polymers in mucoadhesive platforms, a fast-screening in vitro adhesion test is desirable. Methods:Commercial oral films were tested using Texture Analyzer CT3-1000g by mounting them on the bottom of the TA10 probe using VHB-double sided acrylic tape. The maximum negative detachment force was recorded against various substrates (agar 5% and filter papers) in the presence of artificial saliva (pH 6.8) in compression mode. The effect of test parameters on the adhesive force were investigated. Results:Compared to filter paper, agar did not offer reproducible results. Target force of 100g resulted in adequate contact of samples with substrates. Artificial saliva volume of 200 μl found to sufficiently hydrate (but not to dissolve) the films and express adhesion. Hold time of 15 sec was found desirable as longer hold time resulted in the dissolution of some films and shorter hold time did not provide enough hydration. Test speed of 0.1mm/sec generated more reproducible adhesive forces. Conclusion:An in vitro adhesion test was developed and was able to differentiate between various mucoadhesive film platforms. Grants:This study was supported by NSU Grant#335114.

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Feb 21st, 8:30 AM Feb 21st, 4:00 PM

Development of an In Vitro Adhesion Test to Screen Mucoadhesive Polymeric Drug Carriers Using Texture Analyzer

Nova Southeastern University, Davie, Florida, USA

Objective:Developing an in vitro adhesion test to evaluate mucoadhesive oral films using texture analyzer. Background:Adhesive polymeric excipients are the integral part of many oral and topical dosage forms. These are used to increase retention of the drug at the site of application or in the gastrointestinal tract enhancing drug absorption and half-life. Ex vivo tests on animal tissues are commonly used to characterize mucoadhesion, however it is hard and costly. To differentiate adhesive properties of such polymers in mucoadhesive platforms, a fast-screening in vitro adhesion test is desirable. Methods:Commercial oral films were tested using Texture Analyzer CT3-1000g by mounting them on the bottom of the TA10 probe using VHB-double sided acrylic tape. The maximum negative detachment force was recorded against various substrates (agar 5% and filter papers) in the presence of artificial saliva (pH 6.8) in compression mode. The effect of test parameters on the adhesive force were investigated. Results:Compared to filter paper, agar did not offer reproducible results. Target force of 100g resulted in adequate contact of samples with substrates. Artificial saliva volume of 200 μl found to sufficiently hydrate (but not to dissolve) the films and express adhesion. Hold time of 15 sec was found desirable as longer hold time resulted in the dissolution of some films and shorter hold time did not provide enough hydration. Test speed of 0.1mm/sec generated more reproducible adhesive forces. Conclusion:An in vitro adhesion test was developed and was able to differentiate between various mucoadhesive film platforms. Grants:This study was supported by NSU Grant#335114.