Faculty Articles

Title

Improved immobilization of gelatin on a modified polyurethane urea

ISBN or ISSN

1530-8030

Publication Title

Journal of Bioactive and Compatible Polymers

Volume

30

Issue

1

Publication Date / Copyright Date

1-1-2015

First Page

57

Last Page

73

Publisher

Sage Publications

DOI Number

10.1177/0883911514558368

Abstract

In this study, polyurethane urea was surface-modified to elevate cell recognition through immobilization of bioactive gelatin. The poly(urethane urea) was synthesized using poly(ε-caprolactone) diol in the absence of a chain extender. The synthesized polyurethane urea was then functionalized with gelatin (gelatin-grafted poly(urethane urea)) via aminolysis. Chemical changes at the polyurethane urea surface were monitored using titration, water contact angle. Fourier transform infrared, and zeta potential measurements. Significantly larger amounts of gelatin were grafted on the polyurethane urea surface compared to those previously reported for poly(ε-caprolactone) diol (three times more) and polyurethanes (two times more), while the mechanical properties were not compromised. Proliferation of human adipose–derived mesenchymal stem cells on the polyurethane urea and the gelatin-grafted polyurethane urea was evaluated through MTT assay. Although both samples enhanced human adipose–derived mesenchymal stem cells’ proliferation, gelatin-grafted polyurethane urea supported human adipose–derived mesenchymal stem cells’ proliferation at a remarkably higher rate.

Disciplines

Medicine and Health Sciences | Pharmacy and Pharmaceutical Sciences

Keywords

Polyurethane urea, aminolysis, biomolecule immobilization, grafting density, bioactivity, human adipose–derived mesenchymal stem cells

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