Novel flow-cytometric method for separating cell types in differentiated F9 embryoid bodies
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The differentiation of F9 teratocarcinoma cells mimics the formation of a mouse embryonic tissue, the primitive endoderm. In vitro, small aggregates of F9 cells, termed embryoid bodies, differentiate in response to retinoic acid and develop a surface epithelium that is characterized by the production of alpha-fetoprotein. In the present study, cellular autofluorescence profiles obtained by fluorescence-activated embryoid bodies were composed of a single type of cell. In contrast, retinoic acid-induced embryoid bodies were composed of two cell types: a major population displaying autofluorescence levels similar to those of cells from undifferentiated embryoid bodies and a second population displaying higher autofluorescence. RNA analyses demonstrated that the transcription of alpha-fetoprotein was associated only with the more highly autofluorescent population, indicating that flow cytometry provides a novel mechanism for the separation of undifferentiated cells from differentiated endoderm cells in F9 embryoid bodies.
Medicine and Health Sciences | Pharmacy and Pharmaceutical Sciences
Animals, Antibodies, Cell Differentiation, Cell Line, Cell Separation, Flow Cytometry, Fluorescein-5-isothiocyanate, Fluorescent Dyes, Immunohistochemistry, Mice, RNA, RNA, Neoplasm, Rabbits, Teratocarcinoma, Tretinoin, Tumor Cells, Cultured, Wheat Germ Agglutinins
Burdsal, C A.; Pedersen, R A.; Hyun, W C.; and Latimer, Jean Johanna, "Novel flow-cytometric method for separating cell types in differentiated F9 embryoid bodies" (1995). College of Pharmacy Faculty Articles. 14.