Localization of the mouse Mcf-2 (Dbl) protooncogene within a conserved linkage group on the mouse X chromosome.
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Cytogenetics and cell genetics
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A mouse cDNA probe homologous to the human MCF2 transforming sequence has been identified and partially cloned, and is used here to localize the gene on the mouse X chromosome. The human gene has been physically mapped to within 60 kb of the gene for coagulation factor IX, within a large conserved linkage group between the mouse and human genomes which extends from HPRT to G6PD on the X chromosomes of both mammalian species. In situ hybridization of the mouse Mcf-2 probe onto mouse metaphase chromosomes indicates that this gene lies in the same region of the X chromosome as Cf-9, the mouse gene for coagulation factor IX. Moreover, segregation of species-specific genomic DNA polymorphisms for Mcf-2 and Cf-9 in a total of 203 individuals derived from two large interspecific mouse backcross populations (which are also segregating for 17 other X-linked molecular markers) demonstrates that the mouse genes are separated by only 0.5 +/- 0.5 cM. Despite this short distance we were able to order Mcf-2 and Cf-9 relative to one another and other genes in this region. The mouse gene order Hprt-Cf-9-Mcf-2-G6pd predicts a similar ordering of genes on the human X chromosome, a gene order which has only recently been demonstrated by physical mapping. Thus, the map location and linkage relationships of the Mcf-2 gene are similar in man and mouse, and this unique protooncogenic locus is part of a conserved linkage group on the mammalian X chromosome.
Medical Specialties | Medicine and Health Sciences | Osteopathic Medicine and Osteopathy
Animals, Base Sequence, Blotting, Southern, Chromosome Mapping, Cloning, Molecular, Crosses, Genetic, Factor IX, Female, Genetic Linkage, Guanine Nucleotide Exchange Factors, Humans, Hypoxanthine Phosphoribosyltransferase, Male, Mice, Molecular Sequence Data, Proto-Oncogene Proteins, Proto-Oncogenes, Sequence Homology, Nucleic Acid, X Chromosome
Grant, S G.; Mattei, M G.; Galland, F; Stephenson, D A.; Keitz, B T.; Birnbaum, D; and Chapman, V M., "Localization of the mouse Mcf-2 (Dbl) protooncogene within a conserved linkage group on the mouse X chromosome." (1990). Faculty Articles. 1524.