A Polyaniline-Based Optical Biosensing Platform Using an Entrapped Oxidoreductase Enzyme
Sensors and Actuators B: Chemical
Glucose monitoring, Optical biosensor, Polyaniline, Glucose oxidase, Oxidoreductase, Gelation
A novel optical biosensing platform utilizing the unique solubility and chemochromic properties of polyaniline is presented. A facile, ion-induced reprecipitation method leads to the entrapment of a chosen oxidoreductase enzyme, which, in the presence of its associated substrate, catalyzes a reversible redox change in the host polymer. This change is monitored via the UV–vis absorption and subsequently analyzed to fit a Michaelis–Menten model. Here, in vitro prototype devices demonstrate selective sensing of glucose, choline, and uric acid, and the potential to be adapted for use as part of real-time in vivo monitoring systems is discussed.
Nemzer, L. R., & Epstein, A. J. (2010). A Polyaniline-Based Optical Biosensing Platform Using an Entrapped Oxidoreductase Enzyme. Sensors and Actuators B: Chemical, 150, (1), 376 - 383. https://doi.org/10.1016/j.snb.2010.06.054. Retrieved from https://nsuworks.nova.edu/cnso_chemphys_facarticles/87