All rights reserved. This publication is intended for use solely by faculty, students, and staff of Nova Southeastern University. No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, now known or later developed, including but not limited to photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author or the publisher.
Thesis - NSU Access Only
M.S. Ocean Science
Gary L. Hitchcock
Jerry T. Thornthwaite
Batch cultures of the cyanobacteria Synechococcus spp. (strains 48 Syn and 2346) and Porphyridium cruentum (Rhodophyta) were grown in nitrogen-limited (N:P=4:l) and phosphorus-limited (N:P=50:l) seawater media. Optical properties, including particle size (forward angle light scatter), particle granularity (right angle light scatter) and relative mean channel red (> 600 nm) and green (510-550 nm) fluorescence were measured for 10 days using a flow cytometer. Dissolved nitrogen (nitrate), phosphorus (phosphate), cell abundance and chlorophyll concentrations were also measured.
Results indicated that phosphorus-limited cultures yield higher chlorophyll concentration, fluorescence and granularity (right angle scatter) values than did nitrogen-limited cultures. Comparison of these samples with a preliminary investigation shows nutrient-rich cultures (N:P=4:l, N:P=50:l) have larger particle size and higher fluorescence values than relatively nutrient-poor cells cultured in f/20 media. Secondary (R2) populations have been determined for all samples, either by light scatter or fluorescence anomalies. Particularly notable was Synechococcus 2346 (phosphorus-limited) which exhibited a secondary population characteristic for more than half of the experiment. Highly fluorescent particles are suggested as either formative daughter cells, cellular “clumping" or a cellular optical response to batch culture turbidity; these particles have a profound influence on the relative refractive index of the culture with time.
Flow cytometric analysis can be an effective tool in the determination of not only differences in the optical properties and fluorescent signatures of various cyanobacterial strains, but also of population variation within a single strain.
Jerome Lynn Hall. 1989. Optical Properties of Marine Phytoplankton: A Study in Multiparameter Flow Cytometry. Master's thesis. Nova Southeastern University. Retrieved from NSUWorks, Oceanographic Center. (364)
To access this thesis/dissertation you must have a valid nova.edu OR mynsu.nova.edu email address and create an account for NSUWorks.
If you are the author of this work and would like to grant permission to make it openly accessible to all, please click the Free My Thesis button.