Development and Characterization of Thirteen Microsatellite Markers for the Longbill Spearfish (Tetrapturus pfluegeri)

Authors

Andrea M. Bernard, Nova Southeastern UniversityFollow
A. W. S. Hilsdorf, Universidade de Mogi das Cruzes - Brazil
A. F. Amorim, Agencia Paulista de Tecnologia dos Agronegocios - BrazilFollow
Rodrigo Rodrigues Domingues, Universidade de Mogi das Cruzes - Brazil
Mahmood S. Shivji, Nova Southeastern UniversityFollow

ResearcherID

G-4080-2013

Document Type

Article

Publication Title

Conservation Genetics Resources

ISSN

1877-7252

Publication Date

9-28-2013

Keywords

Billfish genetics, Billfish conservation and management, Istiophoridae, STR

Abstract

The longbill spearfish (Tetrapturus pfluegeri; Istiophoridae) is an epipelagic billfish species broadly distributed in the Atlantic Ocean and exploited by multinational fisheries. No data exist on the population structure or genetic diversity of this apex predator, despite a strong need for this information to inform international conservation and management efforts. Thirteen microsatellite loci, comprising mostly tri- and tetra-nucleotide repeats, were isolated and characterized for this species by genotyping individuals (n = 29–42) obtained from western North and South Atlantic fisheries. The average number of alleles ranged from 3 to 16 and the expected heterozygosity ranged from 0.35 to 0.86. This suite of markers provides the first population genetic nuclear resources for the longbill spearfish, allowing initiation of studies for assessing the population structure and demography of this poorly understood species.

DOI

10.1007/s12686-013-0038-y

Volume

6

Issue

1

First Page

169

Last Page

171

Comments

©Springer Science+Business Media Dordrecht 2013

Additional Comments

FAPESP grant #: 2009/54.660-6

NSUWorks Citation

Andrea M. Bernard, A. W. S. Hilsdorf, A. F. Amorim, Rodrigo Rodrigues Domingues, and Mahmood S. Shivji. 2013. Development and Characterization of Thirteen Microsatellite Markers for the Longbill Spearfish (Tetrapturus pfluegeri) .Conservation Genetics Resources , (1) : 169 -171. https://nsuworks.nova.edu/occ_facarticles/352.