ACTH4 -10, Substance P, and Dizolcipine (Mk-801) Accelerate Functional Recovery After Hemilabyrinthectomy in Goldfish

Authors

Rosana Mattioli, Universidade Federal de São Carlos - Brazil
Joseph P. Huston, University of Düsseldorf - Germany
Richard E. Spieler, Nova Southeastern UniversityFollow

Document Type

Article

Publication Title

Neural Plasticity

ISSN

2090-5904

Publication Date

2000

Keywords

Goldfish, MK-801, Learning, Substance P, ACTH4- 10, Functional recovery

Abstract

In this study, we evaluated the goldfish model of hemilabyrinthectomy for investigating potential recovery-promoting drugs. In this lesion model, the unilateral removal of the labyrinth induces a postural imbalance in response to light (Dorsal Light Reflex), from which the animals can recover over time. The behavioral effects of two neuropeptides were tested–namely, of substance P and ACTH4-10, both of which are known to promote functional recovery in several other lesion models. Furthermore, the effect of MK- 801, an antagonist of the glutamatergic NMDAreceptor subtype, was tested because this substance has also been shown to exert a neuroprotective effect. After lesion of the right labyrinth, the animals (n=12) were treated intraperitoneally daily either with vehicle (n=12), substance P (n=11) , ACTH4-10 (n=12) , or MK- 801 (n=12) . Another group(n=11) , which served as a non-lesion control, did not receive hemilabyrinthectomy or systemic injections. The lesion group, treated post-operatively with vehicle, did not recover from the postural deviation over the 24-d testing period. In contrast, all three test substances accelerated the functional recovery after unilateral labyrinthectomy. The decrease of the dorsal light reflex persisted even after cessation of drug treatment after 20d. The results indicate that using the dorsal light reflex in the model of hemilabyrinthectomy in goldfish provides a useful approach to studying the ability of potential new neurotrophic or neuroprotective drugs to promote functional recovery.

DOI

10.1155/NP.2000.291

Volume

7

Issue

4

First Page

291

Last Page

301

Comments

©Freund & Pettman, U.K., 2000

Additional Comments

FAPESP Brazil grant #: 95/4337-0; Deutsche Forschungsgemeinschaft grant #: HU 306/13-1

NSUWorks Citation

Rosana Mattioli, Joseph P. Huston, and Richard E. Spieler. 2000. ACTH4 -10, Substance P, and Dizolcipine (Mk-801) Accelerate Functional Recovery After Hemilabyrinthectomy in Goldfish .Neural Plasticity , (4) : 291 -301. https://nsuworks.nova.edu/occ_facarticles/165.