Faculty Articles

Mouse alpha 1-protease inhibitor is not an acute phase reactant

Publication Title

Archives of biochemistry and biophysics

Publisher

Academic Press

ISSN

0003-9861

Publication Date

4-1-1986

Keywords

Acute-Phase Proteins, Animals, Blood Proteins, DNA, Immunoelectrophoresis, Two-Dimensional, Inflammation, Liver, Male, Mice, Mice, Inbred C57BL, RNA, Messenger, Serpins, Trypsin Inhibitors, alpha 1-Antitrypsin

Abstract

Mouse plasma contains two major protease inhibitors, alpha 1-protease inhibitor (alpha 1-PI) and contrapsin, which have high affinity for bovine trypsin. Systemic injury, such as turpentine-induced inflammation, did not change the plasma concentration of alpha 1-PI, but increased that of contrapsin by 50%. The concentration of hepatic alpha 1-PI mRNA was determined by Northern blot hybridization and was not significantly affected by the acute phase reaction. J.M. Frazer, S.A. Nathoo, J. Katz, T.L. Genetta, and T.H. Finley [1985) Arch. Biochem. Biophys. 239, 112-119) have reported a threefold increase of mRNA for the elastase specific alpha 1-PI but this increase was not demonstrated by the present study. The mRNAs for known mouse acute phase plasma proteins were, however, stimulated severalfold by the same treatment. These results indicate that in the mouse, as opposed to human, alpha 1-PI is not an acute phase reactant.

DOI

10.1016/0003-9861(86)90495-9

Volume

246

Issue

1

First Page

488

Last Page

493

Disciplines

Medicine and Health Sciences | Pharmacy and Pharmaceutical Sciences

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