Department of Anesthesia Faculty Articles
PubMed Identifier
7310697
Publication Title
The Journal of Physiology
Publisher
Cambridge University Press
ISSN
0022-3751
Publication Date
May 1981
Keywords
Neurosciences & Neurology, Physiology
Abstract
1. Isolated Amphiuma small intestine exposed on both surfaces to buffered or unbuffered media generated gradients of pH under short-circuited conditions consistent with secretion of HCO3(-). 2. When unbuffered mucosal medium was maintained at pH 7.4 by addition of acid, alkalinization of the mucosal medium occurred at a rate of 1-2 microequiv/hr cm2 under short-circuit conditions (Isc) and was reduced by anoxia, acetazolamide or removal of CO2. 3. The rate of HCO3(-) secretion (JHCO3(-)) was reduced at a mucosal pH above or below 7.4 and was proportional to serosal HCO3(-). 4. JHCO3(-) was reduced in Na+-free (choline) and Cl-free (SO4(2-) media and after exposure to the stilbene SITS. 5. The difference JHCO3(-)--Isc was consistent with net Cl- absorption. 6. The tissue resistance (Rt) was elevated upon exposure to serosal HCO3(-) and lowered by mucosal HCO3(-). 7. The intestinal mucosa exhibited carbonic anhydrase activity that was sensitive to ethoxazolamide. 8. It is concluded that HCO3(-) secretion is active, influenced by intracellular carbonic anhydrase activity and coupled to Cl- and possibly Na+ absorption.
DOI
https://doi.org/10.1113/jphysiol.1981.sp013717
Volume
314
First Page
429
Last Page
443
Funding Information
AM 00367/AM/NIADDK NIH HHS/United States AM 17361/AM/NIADDK NIH HHS/United States AM 26870/AM/NIADDK NIH HHS/United States
PubMed ID
7310697
Disciplines
Anesthesiology
NSUWorks Citation
Imon, Michael A. and White, John F., "Intestinal Bicarbonate Secretion in Amphiuma Measured by PH Stat in Vitro: Relationship with Metabolism and Transport of Sodium and Chloride Ions" (1981). Department of Anesthesia Faculty Articles. 7.
https://nsuworks.nova.edu/hpd_anes_facarticles/7
