Department of Anesthesia Faculty Articles
PubMed Identifier
6837745
A Role for Basolateral Anion Exchange in Active Jejunal Absorption of Hco-3
Publication Title
The American Journal of Physiology
Publisher
American Physiological Society
ISSN
0002-9513
Publication Date
April 1983
Keywords
Physiology, Zoology, Nutrition and dietetics, Anatomy and morphology, Whole animal physiology, Ion and water relations, Nutrition, Digestion, Digestive system, Intestine
Abstract
Isolated short-circuited segments of jejunum from Amphiuma absorbed HCO-3 at a rate determined by the anion composition of the bathing media. The rate of HCO-3 absorption (JHCO-3) measured by pH-stat was high when the major anion was Cl-, Br-, I-, or SO2-(4) but was lower when gluconate or benzene sulfonate (SO-3) was the anion. The disulfonic stilbenes SITS, DIDS, and DNDS at 1 mM reduced JHCO-3 and short-circuit current when added to the serosal bathing medium. Inhibition by SITS was comparable whether Cl-, Br-, or SO2-(4) was the major anion, and SITS produced a small inhibition of JHCO-3 in gluconate-based media and no effect in benzene SO3-based media. Under conditions in which tissue conductance was low SITS lowered tissue conductance further. In Cl- media SITS reduced short-circuit current consistent with inhibition of Cl- absorption as well as HCO-3 absorption. Inhibition of the Cl- current by DIDS was not reversed by washout, although the inhibition of JHCO-3 was reversed. The rate of HCO-3 absorption in gluconate media could be increased by serosal addition of Cl-, Br-, or I-. It is concluded that the process that results in net jejunal HCO-3 absorption entails anion-HCO-3 exchange at the basolateral membrane and is distinct from the basolateral Cl(-)-HCO-3 exchange process involved in Cl- absorption.
DOI
https://doi.org/10.1152/ajpgi.1983.244.4.g397
Volume
244
Issue
4
First Page
G397
Last Page
405
Funding Information
AM-00367/AM/NIADDK NIH HHS/United States AM-17361/AM/NIADDK NIH HHS/United States
Disciplines
Anesthesiology
NSUWorks Citation
White, John F. and Imon, Michael A., "A Role for Basolateral Anion Exchange in Active Jejunal Absorption of Hco-3" (1983). Department of Anesthesia Faculty Articles. 3.
https://nsuworks.nova.edu/hpd_anes_facarticles/3
