Chemistry and Physics Faculty Articles

Title

Enzyme Entrapment in Polyaniline FIlms Observed via Fluorescence Anisotropy and Antiquenching

Document Type

Article

Publication Date

5-10-2014

Keywords

Antiquenching, Biosensor, Fluorescence anisotropy, Enzyme immobilization, Oxidoreductase, Polyaniline

Publication Title

Modern Physics Letters B

ISSN

0217-9849

Volume

28

Issue/No.

11

First Page

1

Last Page

12

Peer Reviewed

1

Abstract

The facile entrapment of oxidoreductase enzymes within polyaniline polymer films by inducing hydrophobic collapse using phosphate buffered saline (PBS) has been shown to be a cost-effective method for fabricating organic biosensors. Here, we use fluorescence anisotropy measurements to verify enzyme immobilization and subsequent electron donation to the polymer matrix, both prerequisites for an effective biosensor. Specifically, we measure a three order of magnitude decrease in the ratio of the fluorescence to rotational lifetimes. In addition, the observed fluorescence antiquenching supports the previously proposed model that the polymer chain assumes a severely coiled conformation when exposed to PBS. These results help to empirically reinforce the theoretical basis previously laid out for this biosensing platform.

DOI

10.1142/S021798491430004X

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