Chemistry and Physics Faculty Articles

Title

A Polyaniline-Based Optical Biosensing Platform Using an Entrapped Oxidoreductase Enzyme

Document Type

Article

Publication Date

2010

Keywords

Glucose monitoring, Optical biosensor, Polyaniline, Glucose oxidase, Oxidoreductase, Gelation

Publication Title

Sensors and Actuators B: Chemical

ISSN

0925-4005

Volume

150

Issue/No.

1

First Page

376

Last Page

383

Peer Reviewed

1

Abstract

A novel optical biosensing platform utilizing the unique solubility and chemochromic properties of polyaniline is presented. A facile, ion-induced reprecipitation method leads to the entrapment of a chosen oxidoreductase enzyme, which, in the presence of its associated substrate, catalyzes a reversible redox change in the host polymer. This change is monitored via the UV–vis absorption and subsequently analyzed to fit a Michaelis–Menten model. Here, in vitro prototype devices demonstrate selective sensing of glucose, choline, and uric acid, and the potential to be adapted for use as part of real-time in vivo monitoring systems is discussed.

Comments

©2010 Elsevier B.V. All rights reserved.

Additional Comments

IGERT grant #: 0221678; NS EC grant #: EEC-0425626

DOI

10.1016/j.snb.2010.06.054

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