Expression of the Human C-Fms Proto-Oncogene in Hematopoietic Cells and its Deletion in the 5q− Syndrome
The c-fms proto-oncogene was shown to be expressed in human bone marrow and in differentiated blood mononuclear cells, suggesting that its gene product plays a role in hematopoietic maturation. The c-fms mRNA was not detected in HL-60 cells, an established promyelocytic line, whereas c-fms expression appeared 48 hr after induction when most cells had differentiated into macrophages. An acquired deletion of chromosome 5 (5q−) in bone marrow cells is associated with abnormalities in blood cell production. The normal 5 and 5q− chromosomes were segregated by construction of cell hybrids between bone marrow and rodent cells. A selective system was used that requires retention of the structural gene for dihydrofolate reductase, located on human chromosome 5. Analysis of DNA from individual hybrid clones revealed that the 5q− deletion had removed the c-fms gene. We postulate that hemizygosity at the c-fms locus leads to abnormalities in hematopoietic maturation.
Nienhuis, Arthur W.; H. Franklin Bunn; Patricia H. Turner; T. Venkat Gopal; William Nash; Stephen J. O'Brien; and Charles J. Sherr. 1985. "Expression of the Human C-Fms Proto-Oncogene in Hematopoietic Cells and its Deletion in the 5q− Syndrome." Cell 42, (2): 421-428. http://nsuworks.nova.edu/cnso_bio_facarticles/385