Biology Faculty Articles

Title

Nanoparticles for Fidgety Cell Movement and Enhanced Wound Healing

Document Type

Article

Publication Date

9-2015

Publication Title

Journal of Investigative Dermatology

Keywords

Cell Motility, Wound Healing, Microtubules, Cell Mitigation, Dermatology

ISSN

0022-202X

Volume

135

Issue/No.

9

First Page

2151

Last Page

2153

Abstract

Complex spatiotemporal interaction of Rho GTPases with microtubules (MTs) and MT-associated proteins drives directed cellular migration. In this issue, Charafeddine et al. describe a role for a novel MT-severing enzyme, fidgetin-like 2 (FL2), in directional migration of keratinocytes and fibroblasts. FL2 normally localizes to the leading edge of the cell cortex where it shears MTs, thus dictating the size and distribution of focal adhesions by regulating cytoskeletal remodeling. Small interfering RNA (siRNA)-directed knockdown of FL2 increases cell migration and focal adhesion area in vitro through possible interaction with Rho GTPases. Efficient FL2 knockdown in murine wounds was achieved using nanoparticles as a siRNA delivery vehicle, and this resulted in enhanced wound closure in vivo. Effective siRNA nanoparticle targeting of MT-severing enzymes offers promise of controlled and targeted delivery that may maximize therapeutic success for patients with burn wounds and chronic wound disorders.

Comments

Copyright of Journal of Investigative Dermatology is the property of Nature Publishing Group and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use.

This document is currently not available here.

Peer Reviewed

Find in your library

Share

COinS