Title

Manganese Oxide Particles as Cytoprotective, Oxygen Generating Agents

Document Type

Article

Publication Date

9-1-2017

Publication Title

Acta Biomaterialia

Keywords

Nanoparticles, Cytoprotection, Cell culture, In vitro oxygen generation, Cell encapsulation, Reactive oxygen species

ISSN

1742-7061

Volume

59

First Page

327

Last Page

337

Abstract

Cell culture and cellular transplant therapies are adversely affected by oxidative species and radicals. Herein, we present the production of bioactive manganese oxide nanoparticles for the purpose of radical scavenging and cytoprotection. Manganese comprises the core active structure of somatic enzymes that perform the same function, in vivo. Formulated nanoparticles were characterized structurally and surveyed for maximal activity (superoxide scavenging, hydrogen peroxide scavenging with resultant oxygen generation) and minimal cytotoxicity (48-h direct exposure to titrated manganese oxide concentrations). Cytoprotective capacity was tested using cell exposure to hydrogen peroxide in the presence or absence of the nanoparticles. Several ideal compounds were manufactured and utilized that showed complete disproportionation of superoxide produced by the xanthine/xanthine oxidase reaction. Further, the nanoparticles showed catalase–like activity by completely converting hydrogen peroxide into the corresponding concentration of oxygen. Finally, the particles protected cells (murine β-cell insulinoma) against insult from hydrogen peroxide exposure. Based on these observed properties, these particles could be utilized to combat oxidative stress and inflammatory response in a variety of cell therapy applications.

Comments

©2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

DOI

10.1016/j.actbio.2017.07.006

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