Marine & Environmental Sciences Faculty Articles
Document Type
Article
Publication Date
3-5-2015
Publication Title
Microbiology Open
Keywords
16S rRNA, Bacterioplankton, Coral reef, South Florida
ISSN
2045-8827
Volume
4
Issue/No.
3
First Page
390
Last Page
408
Abstract
Coastal waters adjacent to populated southeast Florida possess different habitats (reefs, oceanic inlets, sewage outfalls) that may affect the composition of their inherent microbiomes. To determine variation according to site, season, and depth, over the course of 1 year, we characterized the bacterioplankton communities within 38 nearshore seawater samples derived from the Florida Area Coastal Environment (FACE) water quality survey. Six distinct coastal locales were profiled – the Port Everglades and Hillsboro Inlets, Hollywood and Broward wastewater outfalls, and associated reef sites using culture-independent, high-throughput pyrosequencing of the 16S rRNA V4 region. More than 227,000 sequences helped describe longitudinal taxonomic profiles of marine bacteria and archaea. There were 4447 unique operational taxonomic units (OTUs) identified with a mean OTU count of 5986 OTUs across all sites. Bacterial taxa varied significantly by season and by site using weighted and unweighted Unifrac, but depth was only supported by weighted Unifrac, suggesting a change due to presence/absence of certain OTUs. Abundant microbial taxa across all samples included Synechococcus, Pelagibacteraceae, Bacteroidetes, and various Proteobacteria. Unifrac analysis confirmed significant differences at inlet sites relative to reef and outfalls. Inlet-based bacterioplankton significantly differed in greater abundances of Rhodobacteraceae and Cryomorphaceae, and depletion of SAR406 sequences. This study also found higher counts of Firmicutes, Chloroflexi, and wastewater associated SBR1093 bacteria at the outfall and reef sites compared to inlet sites. This study profiles local bacterioplankton populations in a much broader context, beyond culturing and quantitative PCR, and expands upon the work completed by the National Oceanic and Atmospheric Administration FACE program.
Additional Comments
Supporting Information for this Article can be found here:
http://onlinelibrary.wiley.com/doi/10.1002/mbo3.245/suppinfo
NSUWorks Citation
Alexandra Mandina Campbell, Jay Fleisher, Christopher D. Sinigalliano, James R. White, and Jose V. Lopez. 2015. Dynamics of Marine Bacterial Community Diversity of the Coastal Waters of the Reefs, Inlets, and Wastewater Outfalls of Southeast Florida .Microbiology Open , (3) : 390 -408. https://nsuworks.nova.edu/occ_facarticles/486.
ORCID ID
0000-0002-1637-4125
ResearcherID
F-8809-2011
DOI
10.1002/mbo3.245
Included in
Genetics and Genomics Commons, Marine Biology Commons, Oceanography and Atmospheric Sciences and Meteorology Commons
Comments
©2015 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use,distribution and reproduction in any medium, provided the original work is properly cited.