Presentation Title

Time-Dependent Effects of 5.25 % NaOCl and MTAD on the Calcium Content of Root Dentin

College

College of Dental Medicine

Location

Signature Grand, Davie, Florida, USA

Format

Poster

Start Date

25-4-2008 12:00 AM

End Date

25-4-2008 12:00 AM

Abstract

Objectives. The aim of this study is to evaluate the time-dependent effects of 5.25% NaOCl with or without subsequent MTAD (Dentsply, Tulsa Dental, OK, U.S.A.) application on the calcium content of root canal dentine. Methodology: Crowns of single-rooted teeth were removed and the roots were bisected longitudinally. The pulp remnants were removed with a toothbrush. The root halves (n = 35) were covered with two layers of nail varnish, leaving the root canal surface exposed. The specimens were, then, dehumidified in a sterilizator for 45min at 120oC. Except one group (5-min MTAD treatment alone), all specimens were immersed in either 5.25% NaOCl or distilled water for 5, 10 or 15 min. Specimens treated with three different application times of 5.25% NaOCl were further exposed to MTAD treatment (5 min). The amount of calcium release from dentin following single (MTAD, NaOCl or Distilled water alone) and combined (NaOCl+MTAD) treatment regimens were determined by flame photometry. The calcium release in groups subjected to single solution treatments were analyzed statistically for each immersion period (1, 5 and 10 min) using Kruskal-Wallis Analysis of Variance. Groups subsequently treated with NaOCl, MTAD and distilled water were statistically subjected to Wilcoxon Signed Rank test for the three immersion periods. Kruskal-Wallis Analysis of Variance and Wilcoxon signed ranks tests were used for statistical analysis of data (p = 0.05). Results. Regardless of treatment time, all “single” and “combined” treatment regimens removed significantly more calcium than that of control (p < 0.05). 5.25 % NaOCl +MTAD combined treatment groups removed significantly more calcium than their single versions, regardless of treatment time (p < 0.05). Conclusions. Subsequent irrigation with MTAD significantly increases the liberation of calcium ions from root canal dentin treated with 5.25% NaOCl for 5, 10 and 15 minutes.

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Apr 25th, 12:00 AM Apr 25th, 12:00 AM

Time-Dependent Effects of 5.25 % NaOCl and MTAD on the Calcium Content of Root Dentin

Signature Grand, Davie, Florida, USA

Objectives. The aim of this study is to evaluate the time-dependent effects of 5.25% NaOCl with or without subsequent MTAD (Dentsply, Tulsa Dental, OK, U.S.A.) application on the calcium content of root canal dentine. Methodology: Crowns of single-rooted teeth were removed and the roots were bisected longitudinally. The pulp remnants were removed with a toothbrush. The root halves (n = 35) were covered with two layers of nail varnish, leaving the root canal surface exposed. The specimens were, then, dehumidified in a sterilizator for 45min at 120oC. Except one group (5-min MTAD treatment alone), all specimens were immersed in either 5.25% NaOCl or distilled water for 5, 10 or 15 min. Specimens treated with three different application times of 5.25% NaOCl were further exposed to MTAD treatment (5 min). The amount of calcium release from dentin following single (MTAD, NaOCl or Distilled water alone) and combined (NaOCl+MTAD) treatment regimens were determined by flame photometry. The calcium release in groups subjected to single solution treatments were analyzed statistically for each immersion period (1, 5 and 10 min) using Kruskal-Wallis Analysis of Variance. Groups subsequently treated with NaOCl, MTAD and distilled water were statistically subjected to Wilcoxon Signed Rank test for the three immersion periods. Kruskal-Wallis Analysis of Variance and Wilcoxon signed ranks tests were used for statistical analysis of data (p = 0.05). Results. Regardless of treatment time, all “single” and “combined” treatment regimens removed significantly more calcium than that of control (p < 0.05). 5.25 % NaOCl +MTAD combined treatment groups removed significantly more calcium than their single versions, regardless of treatment time (p < 0.05). Conclusions. Subsequent irrigation with MTAD significantly increases the liberation of calcium ions from root canal dentin treated with 5.25% NaOCl for 5, 10 and 15 minutes.