Retinoic Acid Inhibition of Serum-Induced C-Fos Transcription in a Fibrosarcoma Cell Line.
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American Association for Cancer Research
We investigated the mechanism by which retinoic acid causes growth arrest and flat reversion of SSV-NRK, simian sarcoma virus-transformed normal rat kidney cells. Northern analysis revealed that both chronic (7 days) and acute (6 h) retinoic acid treatment of serum-stimulated SSV-NRK cells caused a 6-fold decrease in c-fos mRNA levels. In addition, nuclear run-on experiments showed that retinoic acid regulated c-fos expression in SSV-NRK cells at the transcriptional initiation level. Attenuation of c-fos transcription was equal in both retinoic acid-treated and control cells, and no increased c-fos mRNA turnover was detected in retinoic acid-treated cells. Furthermore, there was no observed change in the c-fos mRNA levels after only 30 min of retinoic acid treatment, suggesting that a mechanism involving the interruption of the signal transduction mechanism at the membrane level is unlikely. Because it has been shown that c-fos expression plays a pivotal role in mitogenesis of quiescent fibroblasts, we conclude that the retinoic acid-mediated down-regulation of c-fos expression is a mechanism for growth inhibition in SSV-NRK cells.
Medicine and Health Sciences
Cell Division, Cycloheximide, Down-Regulation, Fibrosarcoma, Neoplastic, Genes, fos, Half-Life, RNA, Signal Transduction, Transcription, Genetic, Tumor Cells, Cultured
Jaffey, Pamela B. M.D.; Chan, Lee-Nien L.; Shao, Junyi; Schneider-Schaulies, Jurgen; and Chan, The-Sheng, "Retinoic Acid Inhibition of Serum-Induced C-Fos Transcription in a Fibrosarcoma Cell Line." (1992). Department of Health Sciences Faculty Articles. 205.