Faculty Books and Book Chapters

Assays of Adrenal GPCR Signaling and Regulation: Measuring Adrenal Beta-arrestin Activity in vivo through Plasma Membrane Recruitment

Title

Assays of Adrenal GPCR Signaling and Regulation: Measuring Adrenal Beta-arrestin Activity in vivo through Plasma Membrane Recruitment

ISBN or ISSN

9780128133194

Chapter

6

Publication Date / Copyright Date

2017

First Page

79

Last Page

87

Publisher

Elsevier Academic Press

Abstract

Production and secretion of catecholamines and aldosterone from the adrenal gland are regulated by various adrenal G protein-coupled receptors (GPCRs), including α2-adrenergic (auto)receptors (ARs) and angiotensin receptors. These hormones, in turn, regulate cardiac function and play important pathophysiological roles in heart failure (HF). Recent studies have identified the GPCR adapter proteins β-arrestins, specifically β-arrestin1, as an important mediator of the GPCR-dependent production of these hormones in the adrenal gland. Upon GPCR agonist activation, β-arrestins translocate from the cytoplasm to the plasma membrane to interact with the phosphorylated and active receptor and mediate its G protein-independent signaling. Here, we describe a simple and fast Western blotting-based technique for measuring adrenal β-arrestin plasma membrane recruitment in vivo, as an (indirect) index of its activity toward various agonist-activated adrenal GPCRs in vivo.

Disciplines

Medicine and Health Sciences | Pharmacy and Pharmaceutical Sciences

Keywords

adrenal gland, G protein-coupled receptors, plasma membrane recruitment

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Assays of Adrenal GPCR Signaling and Regulation: Measuring Adrenal Beta-arrestin Activity in vivo through Plasma Membrane Recruitment
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