Nucleic Acid Profiling in Tumor Exosomes
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Elsevier Academic Press
Exosomes are extracellular membrane vesicles with endocytic origin, and recent studies have demonstrated the spread of oncogenes by exosomes from tumor cells. Hence, the cargo content in the exosomes is of great significance. RNA (coding and noncoding) component of exosomes (evRNA) is of particular diagnostic interest because this provides a message to the neighboring cell, specific for a tumor type. Several methods can characterize such evRNA-based message from tumor cells, and here we discuss a brief overview of such techniques including next-generation sequencing and compare/contrast them for their applications. We discuss some of the caveats and artifacts associated with such techniques including library preparation and present some of ways to overcome such challenges. Lastly, we present some of the potential aspects for the field of evRNA characterization especially at the single EV level. Our main goal is to allow the user for informed selection of a tool for their specific application.
Medicine and Health Sciences | Pharmacy and Pharmaceutical Sciences
evRNA, nucleic acid profiling, tumor exosomes
Trivedi, Malav Suchin and Abreu, Maria, "Nucleic Acid Profiling in Tumor Exosomes" (2018). Faculty Books and Book Chapters. 10.